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中国防痨杂志 ›› 2011, Vol. 33 ›› Issue (9): 592-595.

• 论著 • 上一篇    下一篇

发光二极管荧光显微镜在基层应用的多中心研究


夏辉 赵冰 李强 欧喜超 王胜芬 赵雁林   

  1. 中国疾病预防控制中心结核病预防控制中心国家结核病参比实验室
  • 收稿日期:2011-06-25 出版日期:2011-09-10 发布日期:2012-01-29
  • 通信作者: 赵雁林 E-mail:Zhaoyanlin@chinatb.org
  • 基金资助:

    中国卫生部与比尔及梅琳达·盖茨基金会结核病防治项目(2009-04-01)

Multicenter study of application of fluorescent-staining light-emitting diodes fluorescence microscope at county level in China

XIA Hui, ZHAO Bing, LI Qiang, OU Xi-chao, WANG Sheng-fen, ZHAO Yan-lin   

  1. National Tuberculosis Reference Laboratory, National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention
  • Received:2011-06-25 Online:2011-09-10 Published:2012-01-29
  • Contact: ZHAO Yan-lin E-mail:Zhaoyanlin@chinatb.org

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摘要： 目的  评价荧光染色发光二极管荧光显微镜涂片镜检（简称LED）在基层实验室的应用情况。方法  对研究期间到6个县(区)级结核病防治机构（简称结防机构）就诊的肺结核可疑症状者3691例和随访患者2509例，以及8例没有记录初诊随访患者的共11 288份痰标本进行涂片镜检，每份标本涂2张片，分别使用萋尼（简称Z-N）和LED法进行染色、镜检，比较两种方法在阳性检出率及读片时间等方面的差异。结果  LED法的阳性涂片检出率为11.20%（1264/11 288），较Z-N法（8.59%，970/11 288）提高2.61%（χ2=8086.74，P=0.000）。读片时间LED ［（120.03±38.88）s］明显短于Z-N法［（206.31±75.86）s］（t=28.12，P=0.00）。结论  基层实验室使用LED较Z-N能够提高涂片镜检的阳性检出率，且读片时间明显缩短，在我国基层实验室应用具有较好前景。

关键词: 结核, 肺/诊断, 显微镜检查, 荧光, 多中心研究

Abstract: Objective To assess the application status of fluorescent-staining light-emitting diodes fluorescence microscope (LED) in laboratories at county level in China. Methods Two smears were prepared for each sputum from 11288 specimens coming from 3691 pulmonary tuberculosis suspects, 2509 follow-up patients and 8 cases without record of diagnosis or follow-up. Each of smears were followed with Zeihl-Neelsen (Z-N) and LED respectively. The comparison of smear positive detection rate and reading time between two methods were made. Results The smear positive detection rate using LED were 11.20% (1264/11288), 2.61% higher than that of Z-N (8.59%, 970/11288) (χ2=8086.74, P=0.000). The mean reading time with LED (120.03±38.88)s was significantly shorter than that with Z-N (206.31±75.86)s (t=28.12, P=0.00).Conclusion LED method could increase smear positive detection rate and shorten reading time compared with Z-N, so application of LED at county level laboratories is in prospect.

Key words: Tuberculosis,pulmonary/diagnosis, Microscopy,fluorescence, Multicenter study

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