MIF、TNFα在肺结核组织中表达和意义的初步探讨

摘要/Abstract

摘要： 目的检测MIF、TNF-α在肺结核组织中和正常肺组织中表达的差异，以及2者之间的关系，探讨MIF、TNF-α在肺结核中所起的作用和价值。方法正常肺组17例，肺结核组33例。肺结核组中的样本分类概括为空洞组18例（包含18例结节组和18例结旁组）和无空洞组15例（包含15例结节组和15例结旁组）。用免疫组化技术检测肺标本中蛋白MIF、TNF-α的表达。结果 MIF、TNF-α在肺结核结节中大部分为类上皮和朗汉斯巨细胞表达，其表达明显高于结节旁，结节旁明显高于正常肺组织；且空洞组表达均高于无空洞组。在肺结核结节中MIF和TNF-α呈正相关。结论肺结核患者结核结节中存在MIF、TNF-α的高表达，且2者呈明显正相关。说明MIF、TNF-α与肺结核结节的形成密切相关， MIF与TNF-α在肺结核结节中可能互相诱导、刺激，共同放大炎性反应；且MIF、TNF-α产生过多可能与肺结核空洞的形成有关。

关键词: 结核, 肺, 巨噬细胞游走抑制因子, 肿瘤坏死因子-&alpha

Abstract: Objective To study the expression difference and clinical value of MIF, TNF-α in pulmonary tuberculosis (PTB) tissue and normal lung tissue.  Methods The lung specimens were divided into normal control group (17 cases) and PTB group (33 cases).All the specimens in PTB group were divided into 15 non-cavity groups (including 15 tubercle tissues and 15 peripheral tissues of tubercle) and 18 cavity groups (including 18 tubercle tissues and 18 peripheral tissues of tubercle).The expressions of MIF、TNF-α in lung tussues were examined with quantitative immunohistochemistry assay.  Results There are a lot of expressions of MIF、TNF-α,in which most of them were expressed by epithelioid cell and Langhans’ giant cells in tubercles of PTB. The expression levels of MIF、TNF-α in the tubercles of PTB is much higher than that in the peripheral tissues of the tubercles in PTB, which was much higher than that in the normal lungs. The expression levels of MIF、TNF-α of cavity groups is higher than that of non-cavity groups. The tubercles of PTB, MIF and TNF-α are positively correlated.  Conclusions MIF、TNF-α are closely correlated with the formation of tubercles in PTB. The overexpression of MIF and TNF-α in the tubercles of PTB might play a crucial role in the formation of cavity of PTB.

Key words: tuberculosis, pulmonary, macrophage migration inhibitory factor, tumor necrosis factor-alpha

漆沄石志红苟超伦. MIF、TNFα在肺结核组织中表达和意义的初步探讨[J]. 中国防痨杂志, 2011, 33(3): 153-157.

Qi Yun, Shi Zhihong, Gou Chaolun. The expression and its significance of MIF、TNF-α in pulmonary tuberculosis tissue[J]. Chinese Journal of Antituberculosis, 2011, 33(3): 153-157.

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