布鲁氏菌性脊柱炎动物模型研究进展

doi:10.19982/j.issn.1000-6621.20230163

摘要/Abstract

摘要：

布鲁氏菌病是由布鲁氏菌引起的一种人畜共患传染病。布鲁氏菌集中于脊柱局部侵蚀椎体等组织引发腰背疼痛等相关症状,即为布鲁氏菌性脊柱炎。近年来,布鲁氏菌性脊柱炎发病率增高,临床中漏诊和误诊也时有发生。布鲁氏菌性脊柱炎发病机制较为复杂,目前尚无定论,故临床前实验显得尤为重要,动物模型是基础研究的重要方式,合适的模型研究对临床医生治疗疾病有指导意义。布鲁氏菌性脊柱炎较为复杂,动物模型建立相对困难。作者通过对已建立的动物模型进行分析讨论,以期为后续动物造模研究提供参考。

关键词: 布鲁杆菌病, 脊柱炎, 疾病模型,动物, 综述文献(主题)

Abstract:

Brucellosis is a zoonotic disease caused by Brucella. Brucella concentrated in the local erosion of spine vertebrae and other tissues causes low back pain and other related symptoms, that is, Brucella spondylitis (BS). In recent years, the incidence of BS increased, and missed diagnosis and misdiagnosis occurred from time to time. The pathogenesis of BS is complex, and it has not been accurately identified at present, so the preclinical experiment is particularly important. Animal model is an important way of basic research, and appropriate model research could provide guidance to clinicians for disease treatment. BS is rather complex, and the establishment of animal model is relatively difficult. In this paper, the established animal models are analyzed and discussed in order to provide reference for subsequent animal model research.

Key words: Brucellosis, Spondylitis, Disease models,animal, Review literature as topic

中图分类号:

R516.7

李依奇, 柳永明, 陈耀龙, 杨引君, 刘贝, 温发延, 李岩. 布鲁氏菌性脊柱炎动物模型研究进展[J]. 中国防痨杂志, 2023, 45(9): 913-920. doi: 10.19982/j.issn.1000-6621.20230163

Li Yiqi, Liu Yongming, Chen Yaolong, Yang Yinjun, Liu Bei, Wen Fayan, Li Yan. Research progress on animal model of Brucella spondylitis[J]. Chinese Journal of Antituberculosis, 2023, 45(9): 913-920. doi: 10.19982/j.issn.1000-6621.20230163

图/表 6

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图12~14

表1

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实验动物	造模标准	优点	缺点
小鼠	临床症状、生物发光成像、病理组织切片	成熟较快,遗传背景清楚,价格低廉,易获得	基因敲除后丧失免疫作用,无明显临床症状,病亡率较高,多用于急性布鲁氏菌病研究
兔子	影像学检查、病理组织切片	腰椎解剖结构、病理表现类似人类,易行手术暴露直接感染	难以检测病变时间,缺乏免疫试剂,存在易感性
犬	临床症状、影像学检查、细菌培养	自然状态下感染,出现类似人类感染症状	难以获得,多涉及伦理问题