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中国防痨杂志 ›› 2001, Vol. 23 ›› Issue (4): 241-244.

• 论著 • 上一篇    下一篇

平菇双相培养基用于结核杆菌培养的研究

申建维;袁玮;李娉;刘承丽;兰艳;梅国华;   

  1. 湖北省武汉钢铁(集团)公司第二医院 武汉 430085;
  • 出版日期:2001-04-10 发布日期:2001-11-03

Study of the mushroom biphasic culture’s application on the tuberculosis culture

SHEN Jian wei, YUAN Wei, LI Ping, et al.   

  1. The Second Hospital of Wuhan Iron & Steel (group) Co. Wuhan 430085
  • Online:2001-04-10 Published:2001-11-03

摘要: 目的 以平菇浸出液为基础成分,研制培养结核分支杆菌的一种新型双相培养基。方法 经用H37Rv基础试验及对351份临床标本用酸性罗氏培养基对照。结果 两种培养基总阳性数为172份(49%),其中平菇双相培养基166份(+),阳性检出率为47.3%。酸性罗氏培养基144份(+),阳性检出率为41.03%。分别占总阳性数的96.51%和83.72%,并且大多数结核杆菌在平菇双相培养基上的初生长时间明显快于酸性罗氏培养基时间。结论 结果表明平菇双相培养基成分来源丰富,价格低廉,制作方便,操作简单,适合于我国广大基层医院和结核院所使用。

关键词: 平菇双相培养基, 分支杆菌, 结核, 药敏试验, 培养

Abstract: Objective On the base of mushroom extract, processed a new kind of biphasic culture for tuberculosis’s culture.Methods H37Rv basic test and 351 clinical samples were cultured on both acid Lowenstein-Jensen culture medium and mushroom biphasic culture.Results It shows that the total positive result is 172(49%). Among them, the mushroom biphasic culture’s positive result is 166(47.2%), the acid Lowenstein-Jensen culture medium is 144(41.0%), and the positive rate (of the total positive result) is 96.5% and 83.7%. For the more, the primary grow time on the mushroom biphasic culture is much shorter. Conclusion All shows that mushroom biphasic culture, which is processed conveniently and cheaply, has good practical value in many basic hospitals and tuberculosis hospitals.

Key words: Mushroom biphasic culture, Mycobacterium,tuberculosis, Drug susceptibility test, Culture