全基因组测序在结核病研究中的应用进展

doi:10.3969/j.issn.1000-6621.2018.02.007

摘要/Abstract

摘要：

随着技术的发展及成本的降低,全基因组测序已经广泛应用于结核分枝杆菌的各方面研究当中,包括微进化与传播、宏观进化与种系发生、耐药检测等。根据现有研究成果,全基因组测序已经解决了很多传统分子研究方法无法解决的问题,比如鉴定传播链、分辨复发与再感染、解析结核分枝杆菌的进化过程、快速诊断结核病耐药、诊断混合感染等。尽管如此,目前还存在很多问题亟需解决。笔者对全基因组测序技术的研究方向及成果进行回顾,提出其局限性,并展望其应用前景。

关键词: 分枝杆菌, 结核, 基因组, 细菌, 基因测定, 进化, 分子, 种系发生

Abstract:

With the development of technology and the reduction of cost, whole genome sequencing has been widely used in the research of Mycobacterium tuberculosis (M.tuberculosis) in various respects, including micro-evolution and transmission, macro-evolution and phylogeny, and drug resistance detection. According to the existing research results, genome-wide sequencing has solved many problems that can not be solved by traditional molecular research methods, such as identification of transmission chain, differentiation of recurrent and re-infection, analysis of the evolution of M.tuberculosis, rapid diagnosis of tuberculosis resistance, and diagnosis of mixed infection. Nonetheless, there are still many problems that need to be solved urgently. In this paper, the current research directions and achievements of whole genome sequencing are reviewed, the limitations are proposed, and the future applications are prospected.

Key words: Mycobacterium tuberculosis, Genome, bacterial, Genetic testing, Evolution, molecular, Phylogeny

陈昕昶,张文宏. 全基因组测序在结核病研究中的应用进展[J]. 中国防痨杂志, 2018, 40(2): 149-152. doi: 10.3969/j.issn.1000-6621.2018.02.007

Xin-chang CHEN,Wen-hong ZHANG. Progress in the application of whole genome sequencing in tuberculosis research[J]. Chinese Journal of Antituberculosis, 2018, 40(2): 149-152. doi: 10.3969/j.issn.1000-6621.2018.02.007

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