[1]Cole ST,Brosch R,Parkhill J,et al. Dciphening the biology of M.tubeiculosis from the complete genome sequence.Nature,1998,393(6685):537-544.[2]Cole ST. Learning from the genome sequence of Mycobacterium tuberculosis H37Rv. FEBS Lett,1999,452(1/2):7-10.[3]Orita M, Suzuki Y, Sekiya T, et al. A rapid and sensitive detection of point mutations and genetic polymorphisms using polymerase chain reaction. Genomics, 1989,5(4):874-879.[4]Telenti A,Imboden P,Marchesi F,et al. Direct, automated detection of rifampin-resistant Mycobacterium tuberculosis by polymerase chain reaction and single-strand conformation polymorphism analysis. Antimicrob Agents Chemother, 1993,37(10):2054-2058.[5]Tahmasebi P, Farnia P, Sheikholslami F, et al. Rapid identification of extensively and extremely drug resistant tuberculosis from multidrug resistant strains; using PCR-RFLP and PCR-SSCP. Iran J Microbiol,2012,4(4):165-170.[6]朱敏,李锋,盛国平,等. 应用PCR-SSCP技术检测痰中结核分枝杆菌katG、rpoB、embB基因突变的研究. 中国防痨杂志,2007,29(6):508-510.[7]包洪,于庭,刘爱忠,等. PCR-SSCP方法用于痰标本中结核分支杆菌耐药基因的检测. 中国实验诊断学,2007,11(1):82-84.[8]Fischer SG, Lerman LS. Length-independent separation of DNA restriction fragments in two-dimensional gel electrophoresis. Cell, 1979, 16(1):191-200.[9]Fischer SG, Lerman LS. Separation of random fragments of DNA according to properties of their sequences. Proc Natl Acad Sci U S A, 1980, 77(8):4420-4424.[10]Fischer SG, Lerman LS. DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: correspondence with melting theory. Proc Natl Acad Sci U S A, 1983, 80(6): 1579-1583.[11]Li J,Xin J,Zhang L,et al. Rapid detection of rpoB mutations in rifampin resistant M.tuberculosis from sputum samples by denaturing gradient gel electrophoresis. Int J Med Sci,2012,9(2):148-156.[12]Scarpellini P, Braglia S, Carrera P, et al. Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis. Antimicrob Agents Chemother,1999,43(10):2550-2554.[13]Kiers A, rost AP, van Soolingen D, et al. Use of DNA fingerprinting in international source case finding during a large outbreak of tuberculosis in The Netherlands. Int J Tuberc Lung Dis,1997,1(3):239-245.[14]Zhao LL, Xia Q, Lin N, et al. Multiplex allele-specific PCR combined with PCR-RFLP analysis for rapid detection of gyrA gene fluoroquinolone resistance mutations in Mycobacterium tuberculosis. J Microbiol Methods,2012,88(1):175-178.[15]Caws M, Tho DQ, Duy PM, et al. PCR-restriction fragment length polymorphism for rapid, low-cost identification of isoniazid-resistant Mycobacterium tuberculosis. J Clin Microbiol,2007,45(6):1789-1793.[16]Nachamkin I,Kang C,Weinstein MP. Detection of resistance to isoniazid, rifampin, and streptomycin in clinical isolates of Mycobacterium tuberculosis by molecular methods. Clin Infect Dis,1997,24(5):894-900.[17]Tyagi S, Kramer FR. Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol,1996,14(3):303-308.[18]Saribas Z, Kocagoz T, Alp A, et al. Rapid detection of rifampin resistance in Mycobacterium tuberculosis isolates by heteroduplex analysis and determination of rifamycin cross-resistance in rifampin-resistant isolates. J Clin Microbiol,2003,41(2):816-818.[19]Nam YH,Lee SH,Ahn YC, et al. Detection of rifampin resistant Mycobacterium tuberculosis complex using denaturing HPLC. Korean J Lab Med,2008,28(2):95-102.[20]Cooksey RC, Morlock GP, Holloway BP, et al. Temperature-mediated heteroduplex analysis performed by using denaturing high-performance liquid chromatography to identify sequence polymorphisms in Mycobacterium tuberculosis complex organisms. J Clin Microbiol,2002,40(5):1610-1616.[21]Yip CW, Leung KL, Wong D, et al. Denaturing HPLC for high-throughput screening of rifampicin-resistant Mycobacterium tuberculosis isolates. Int J Tuberc Lung Dis,2006,10(6):625-630.[22]Shi R,Otomo K,Yamada H,et al. Temperature-mediated heteroduplex analysis for the detection of drug-resistant gene mutations in clinical isolates of Mycobacterium tuberculosis by denaturing HPLC, SURVEYOR nuclease. Microbes Infect,2006,8(1):128-135.[23]Evans JT, Hawkey PM, Smith EG,et al. Automated high-throughput mycobacterial interspersed repetitive unit typing of Mycobacterium tuberculosis strains by a combination of PCR and nondenaturing high-performance liquid chromatography. J Clin Microbiol,2004,42(9):4175-4180.[24]Qiu P,Shandilya H,D’Alessio JM,et al. Mutation detection using Surveyor nuclease. Biotechniques,2004,36(4):702-707.[25]Pilato B,De Summa S,Danza K,et al. DHPLC/SURVEYOR nuclease: a sensitive, rapid and affordable method to analyze BRCA1 and BRCA2 mutations in breast cancer families. Mol Biotechnol,2012,52(1):8-15.[26]Williams DL, Spring L, Gillis TP, et al. Evaluation of a polymerase chain reaction-based universal heteroduplex generator assay for direct detection of rifampin susceptibility of Mycobacterium tuberculosis from sputum specimens. Clin Infect Dis,1998,26(2):446-450.[27]Mayta H, Gilman RH, Arenas F, et al. Evaluation of a PCR-based universal heteroduplex generator assay as a tool for rapid detection of multidrug-resistant Mycobacterium tuberculosis in Peru. J Clin Microbiol,2003,41(12):5774-5777.[28]Krothapalli S, May MK, Hestekin CN. Capillary electrophoresis-single strand conformation polymorphism for the detection of multiple mutations leading to tuberculosis drug resistance. J Microbiol Methods,2012,91(1):147-154.[29]Evans JT,Parveen A,Smith GE, et al. Application of denaturing HPLC to rapidly identify rifampicin-resistant Mycobacterium tuberculosis in low-and high-prevalence areas. J Antimicrob Chemother,2009,63(2):295-301.[30]Gundry CN, Vandersteen JG, Reed GH, et al. Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes. Clin Chem,2003,49(3):396-406.[31]Wittwer CT,Reed GH,Gundry CN,et al.High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem,2003,49(6):853-860.[32]Ong DC, Yam WC, Siu GK, et al. Rapid detection of rifampicin- and isoniazid-resistant Mycobacterium tuberculosis by high-resolution melting analysis. J Clin Microbiol,2010,48(4):1047-1054.[33]Yadav R, Sethi S, Mewara A, et al. Rapid detection of rifampicin, isoniazid and streptomycin resistance in Mycobacterium tuberculosis clinical isolates by high-resolution melting curve analysis. J Appl Microbiol,2012,113(4):856-862.[34]Lee AS, Ong DC, Wong JC, et al. High-resolution melting analysis for the rapid detection of fluoroquinolone and streptomycin resistance in Mycobacterium tuberculosis. PLoS One,2012,7(2):e31934.[35]Boehme CC,Nabeta P,Hillemann D, et al. Rapid molecular detection of tuberculosis and rifampin resistance. N Engl J Med,2010,363(11):1005-1015.[36]Martlila HJ,Soini H,Vyshnevskaya E,et al. Line probe assay in the rapid detection of rifampin-resistant Mycobacterium tuberculosis directly from clinical specimens. Scand J Infect Dis,1999,31(3):269-273.[37]Pang Y,Xia H,Zhang Z,et al. Multicenter evaluation of genechip for detection of multidrug-resistant Mycobacterium tuberculosis. J Clin Microbiol,2013,51(6):1707-1713. |