关键词: 分枝杆菌, 非典型性, 生物学鉴定法, 芯片分析技术, 分子探针技术, 寡核苷酸序列分析, 结果与过程评价(卫生保健)

Abstract:

Objective To analyze the results of strains’ identification of clinical isolates of non-tuberculosis mycobacteria (NTM) from three tuberculosis institution in Beijing using chip hybrization (chip method) and 16S rRNA gene sequencing (gene sequencing), and to provided scientific evidences for rapid and accurate diagnosis of clinical mycobacterial disease.Methods One hundred and twenty-nine strains of NTM from 3 institutes in Beijing were performed to identify using chip method and gene sequencing which the blind method was conducted among technicians. The results of two identifications were compared and evaluated.Results Of the 129 strains of NTM, 107 strains were performed by gene sequencing, the identification results were as follows: M.intracellulare in 59 strains (55.1%), M.kansasii in 29 strains (27.1%), M.chelonae/abscessus in 5 strains (4.7%), M.avium in 3 strains (2.8%), M.fortuitum and M.gordonae in each 2 strains (1.9% each), M.xenopi, M.phlei and M.scrofulaceum in each one strain (0.9% each) and other NTM in 4 strains (3.7%). Of 116 strains performed by chip method, 96 strains were performed with both methods. The coincidence rate of the two methods was 88.5% (85/96). There were three foreign strains of NTM identified by gene sequencing, which were M.minnesotense, M.kumamotonense and M.temen.Conclusion The main strains of NTM from three institutes in Beijing are M.intracellulare, M.kansasii and M.chelonae/abscessus. The consistency rate of identification of clinical isolates of NTM by chip method and gene sequencing is high.

Key words: Mycobacteria, atypical, Biological assay, Microchip analytical procedures, Molecular probe techniques, Oligonucleotide array sequence analysis, Outcome and process assessment (health care)