不同感染途径和接种菌量对建立SD大鼠结核菌感染模型的影响

摘要/Abstract

摘要： 目的　探讨建立SD大鼠结核病模型的方法 ,比较不同感染途径和接种菌量在建立SD大鼠结核病动物模型的影响。方法用标准人型结核菌株H₃₇Rv 0.1、1、10 mg 3种接种菌量分别经尾静脉和腹腔途径注入SD大鼠。6周后剖杀大鼠,并比较肺、肝、脾的脏器质量指数,观察肺、肝、脾大体病变和镜下特点,肺、肝、脾的组织匀浆涂片做抗酸染色及结核菌培养。结果肺、肝、脾组织切片HE染色见不同程度增殖性结核结节。随着接种量的增加,其脏器组织病理改变越显著。肺、肝、脾组织切片抗酸染色见炎性结节及多核巨细胞内散乱排列的红色短小抗酸杆菌。接种菌量一致的情况下,尾静脉感染组肺、肝、脾组织病理改变较腹腔感染组显著,肺、肝、脾组织匀浆的结核分枝杆菌培养阳性率较腹腔感染组高（P<0.05）。结论建立SD大鼠肝、脾、肺结核杆菌感染模型,尾静脉感染途径优于腹腔感染途径。SD大鼠对结核菌敏感,可作为结核病实验的动物模型。

关键词: Sprague-Dawley大鼠, 结核, 结核分枝杆菌/微生物学, 动物模型

Abstract: Objective To investigate the effect of pathway and dose of inoculation on establish-ment of experimental tuberculosis in SD rats.Methods Eighty male SD rats were randomized into eight groups.The rats in group C,group D and group E were injected intravenously with 0.1 mg, 1 mg,10mg of Mycobacterium tuberculosis standard strain H37Rv,respectively.The rats of group F,group G and group H were injected intraperitoneally with 0.1mg,1mg,10mg of Mycobacterium tuberculosis standard strain H37Rv,respectively.The rats of group A and group B were injected in- travenously and intraperitoneally with the normal saline,respectively.All rats were sacrificed at six weeks postinfection.Their lungs,livers,and spleens were observed pathologic changes,weighed to compare weight index of organs.The lung,liver,spleen homogenates were cultured for mycobacte- rial growth,smeared and stained to find acid-fast bacilli.Results The histopathological changes in the lungs,livers and spleens showed that macrophages and epithelioid cells aggregated within the tubercle lesion whose periphery are surrounded by lymphocytes.With increasement of dose of inoc- ulation,tubercle lesion change and positive rate of mycobacterial culture of lung,liver and spleen tissues from experimental groups were increasing.Lung,liver,spleen sections with Ziehl-Neelsen staining showed that red,small and short acid-fast bacilli arrayed disorderly within macrophages and inflammatory tubercle.Tubercle lesion change and positive rate of mycobacterial culture of lung,liver,spleen in rats from the groups infected by caudal vein were higher than that from the groups infected by abdominal cavity with the same doses of inoculation.Conclusions Caudal vein pathway is better than abdominal cavity pathway in establishing experimental tuberculosis in SD rat.SD rats can be infected by Mycobacterium tuberculosis and can be used as animal model of ex- perimental tuberculosis.

Key words: Sprague-Dawley rats, Tuberculosis, Mycobacterium tuberculosis/microbiology Animal model

袁小亮,雷建平,李光明,刘翼军,周军,涂少华,薛云平,. 不同感染途径和接种菌量对建立SD大鼠结核菌感染模型的影响[J]. 中国防痨杂志, 2008, 30(2): 90-95.

Yuan Xiaoliang Lei Jianping Li Guangming,et al.. Effect of different pathways and doses of inoculation on establishment of experimental tuberculosis in SD rats[J]. Chinese Journal of Antituberculosis, 2008, 30(2): 90-95.

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