环介导等温扩增法快速检测结核分枝杆菌的初步观察

摘要/Abstract

摘要： 目的建立1种用于结核分枝杆菌快速检测的LAMP技术平台，用于结核分枝杆菌的鉴定。方法使用了11株标准菌株，建立了一套用于结核分枝杆菌快速检测的平台，对灵敏度和特异性进行优化，随后使用47株临床分离株，和13株食源性致病菌对建立的方法进行了验证。结果经过标准菌株建立并优化检测平台，经过优化的LAMP反应体系检出限为1pg的模板DNA，在使用47株临床分离株，和13株食源性致病菌对方法进行验证，发现LAMP特异性为97.4%。结论通过这项研究发现LAMP应用于结核分枝杆菌的快速检测，具有检出浓度低、特异性强和检测周期短的优点。

关键词: 分枝杆菌, 结核, 核酸扩增技术

Abstract: Objective To develop a loop-mediated isothermal amplification (LAMP) method for the detection ofMycobacterium tuberculosis .  Methods 11 standard strains were used to develop the LAMP method in TB detection. The sensitivity and specificity of LAMP were evaluated using 47M. tuberculosisand nontuberculosis mycobacteria isolates and 13 food borne strains.  Results The detection limit of LAMP was 1pg of DNA, the specificity was 97.4%.  Conclusion s The LAMPis a rapid, sensitive, and specific method for the detection ofMycobacterium tuberculosis .

Key words: Mycobacterium tuberculosis, nucleic acid amplification techniques

中图分类号:

R446.5

林世平, 杨应周, 谭卫国, 林一曼, 汪洪富. 环介导等温扩增法快速检测结核分枝杆菌的初步观察[J]. 中国防痨杂志, 2010, 32(8): 52-55.

LIN Shi-Ping, YANG Ying-Zhou, TAN Wei-Guo, LIN Yi-Man, WANG Hong-Fu. Application of LAMP method in rapid detection of Mycobacterium tuberculosis[J]. Chinese Journal of Antituberculosis, 2010, 32(8): 52-55.

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