Table of Content

15 October 2010, Volume 32 Issue 10

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Mycobacterium tuberculosis; drug-resistance; bacterial; microbial sensitivity tests; rifampin; isoniazid;

JIAN Ji-Yong, WANG Nen-Han, YI Jun-Li, XING Qing, LIU Xiao-Xue, WANG Su-Min, HONG Feng

Chinese Journal of Antituberculosis. 2010, 32(10):  9-12. 

Abstract ( 1707 )   PDF (196KB) ( 812 )   Save

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Objective To evaluate the effect of MTBDRplus assay in rapid detection of resistance to rifampin(RIF) and isoniazid(INH) in Mycobacterium tuberculosis clinical isolates collected in Beijing.Methods A total of 169 clinical isolates were enrolled in the study.Using the tranditional drug susceptibility testing as the control,the sensitivity and specificity of the MTBDRplus assay and the variety of gene mutations were assessed.Results The sensitivity and specificity of MTBDRplus assay for the detection of RIF a...

The cross-resistance to levofloxacin and moxifloxacin in Mycobacteriu tuberculosis and gene mutations in gyrA and gyrB

LI Guo-Li, CHEN Peng, SUN Chang-Wen, ZHANG Ling-Xia, LI Bang-Yin, ZHAO Yan-Lin

Chinese Journal of Antituberculosis. 2010, 32(10):  14-19. 

Abstract ( 1961 )   PDF (250KB) ( 1569 )   Save

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Objective To study the cross resistance to levofloxacin(LVF) and moxifloxcin(MXF) in M.tuberculosis(MTB),and to analyze the relationship between gyrA and gyrB gene mutations and the drug resistance level of fluoroquinolones(FQs).Methods The MICs of LVF and MXF in MTB clinical isolates were determined on Lwenstein-Jensen media.The sequences of the gyrA and gyrB genes were determined by PCR-sequencing.Results The MICs of MXF were 2 to 4 times lower than that of LVF.MXF activity against MTB was 2-4 times high...

Research on mycobacteria culture and drug sensitivity assay with microscopic observation method

LIANG Bo-Wen, LU Yu, CHU Nai-Hui

Chinese Journal of Antituberculosis. 2010, 32(10):  20-24. 

Abstract ( 1431 )   PDF (351KB) ( 423 )   Save

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Objective To assess the value of microscopic observation method(MOM) on mycobacteria culture and drug sensitivity assay.Methods The smear-positive sputum specimens were cultured and directly determined the drug-susceptibility test on isoniazid(INH),rifampicin(RFP),streptomycin(SM) and ethambutol(EMB)with MOM,compared with conventional culture on Lǒwenstein-Jensen(L-J) medium and drug susceptibility testing using the absolute concentration method.The inconsistent results were further detected with DNA suquen...

Prokaryotic expression,purification and antigenicity identification of Mycobacterium tuberculosis rv2352c gene

CAO Ting-Ming, JIA Hong-Yan, GU Shu-Xiang, ZHENG Xiao-Jing, LI Zi-Hui, DU Feng-Jiao, LIU Yang, LIU Zhong-Quan, XING Ai-Ying, DU Bo-Ping, MA Yu, ZHANG Zong-De

Chinese Journal of Antituberculosis. 2010, 32(10):  25-30. 

Abstract ( 1285 )   PDF (473KB) ( 413 )   Save

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Objective To express,purify Mycobacterium tuberculosis(M.tb) Rv2352c protein in E.coli and study its antigenicity.Methods The M.tb rv2352c gene was amplified by PCR and then cloned into the expression plasmid pET30a(+).The positive clones were analyzed and transformed into E.coli BL21(DE3).The bacteria expressing Rv2352c were cultured in different concentration of IPTG.The fusion protein was purified by Ni+-NTA column,detected by SDS-PAGE,and reacted with the sera from tuberculosis patients by Western blot....

The Clinical Value of Detecting Tb-DNA in BALF by FQ-PCR in Diagnosing Pulmonary Tuberculosis

LI Zhi-Hui, ZHANG Hong-Man, LIU Jian-Qiang, LIU Peng-Chong, ZHAO Jie, LI Yi, CUI Dan, FENG Xiu-Li

Chinese Journal of Antituberculosis. 2010, 32(10):  32-34. 

Abstract ( 1665 )   PDF (563KB) ( 635 )   Save

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Objective To study the value of detecting M.tuberculosis DNA in bronchoalveolar lavage fluid(BALF) by fluorescent quantitative polymerase chain reaction(FQ-PCR) in diagnosing pulmonary tuberculosis.Methods The BALF from the 187 patients with pulmonary tuberculosis treated first and 41 patients with pneumonia were detected the M.tuberculosis DNA(TB-DNA) by FQ-PCR,and performed mycobacterial culture.The bronchoscopic brushing smears were detected by acid-fast staining.Results The positive rates of detecting B...

Therapeutic effect of Ag85A/B chimeric plasmid DNA vaccine in the mouse model infected by Mycobacterium tuberculosis

LIANG Yan, WU Xue-Qiong, LI Zhong-Ming, LI Ning, ZHANG Jun-Xian, YANG You-Rong, BAI Xue-Juan, YU Qi, HOU Ying, LIU Cui-Hua, AN Hui-Ru, SHI Ying-Chang, LIU Yin-Ping, WANG Bo, WANG Lan, LIU Cheng-Long

Chinese Journal of Antituberculosis. 2010, 32(10):  37-40. 

Abstract ( 1705 )   PDF (456KB) ( 734 )   Save

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Objective To evaluate the therapeutic effect of Ag85A/B chimeric plasmid DNA vaccine in the mouse model infected by M.tuberculosis to establish a new immunotherapeutic agent against the tuberculosis.Methods 50 female BALB/c mice were infected intravenously via the tail vein with 68000 CFU of M.tuberculosis H37Rv,then randomly divided into 5 groups,and treated as follow at the third day after infection:group A,saline|group B,plasmid vector|group C,rifampin|group D,Mycobacterium phlei F.U.36 injection|group E...

The effect of different antigens on enzyme-linked immunospot assay for tuberculosis

MA Li-Ping, LIU Fei, ZHANG Zong-De

Chinese Journal of Antituberculosis. 2010, 32(10):  41-44. 

Abstract ( 1718 )   PDF (191KB) ( 458 )   Save

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Objective To compare the effect of different Mycobacterium tuberculosis antigens on enzyme-linked immunospot assay(ELISPOT).Methods 132 tuberculosis patients and 90 control cases were recruited to the research.The peripheral blood mononuclear cells(PBMC) of all participants were co-cultured with the following antigens: purified protein derivative(PPD),early secretory antigenic target-6(ESAT-6) and ESAT-6/culture filtrate protein-10 fusion protein(ESAT-6/CFP-10,E/C).Spot forming cells(SFC) were enumerated by...

The clinical application of fluorescent quantitative PCR in diagnosis of pulmonary tuberculosis

WU Chi, ZHANG Hong-Mei, ZHAN Neng-Yong, DENG Yong-Cong, DAN Wan-Shui, YU Wei-Ye

Chinese Journal of Antituberculosis. 2010, 32(10):  45-48. 

Abstract ( 1837 )   PDF (197KB) ( 829 )   Save

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Objective To study clinical value of fluorescent quantitative PCR(FQ-PCR) on detection of Mycobacterium tuberculosis complex(MTB).Methods 860 sputum samples from the patients with pulmonary tuberculosis were detected by FQ-PCR,staining and culture,respectively.The sensitivity and specificity of FQ-PCR were assessed by comparing the results.Results The sensitivity of FQ-PCR reached 10~100CFU/ml.The results of other pathogens in respiratory tract were negative by FQ-PCR.The positive rate of FQ-PCR in testing ...

Association of radiographic Features with fasting blood glucose level in diabetic patients with pulmonary tuberculosis

YIN Hong-Yun, LIU Yi-Dian, SHI Xiang, HE Ya, MA Jun, ZHANG Zhong-Shun

Chinese Journal of Antituberculosis. 2010, 32(10):  50-53. 

Abstract ( 1336 )   PDF (198KB) ( 775 )   Save

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Objective To investigate the association of radiographic manifestation with fasting plasma glucose level,duration of diabetes and other factors in patients with diabetes and pulmonary tuberculosis(DM-PTB).Methods(1)86 new patients with PTB and diabetes Mellitus(DM) were enrolled from April 2009 to September 2009,cases with using steroids and immunosuppressive drugs,non-tuberculous mycobacterial disease and HIV positive were excluded.(2)The demographic data,the time of diabetes onset,the methods of diabetes ...

Analysis on 53 patients with pulmonary tuberculosis complicated by MODS

CHEN Yuan-Yuan, ZHU Min

Chinese Journal of Antituberculosis. 2010, 32(10):  54-57. 

Abstract ( 1226 )   PDF (177KB) ( 305 )   Save

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Objective To evaluate the risk factors for motality of patients with pulmonary tuberculosis complicated by multiple organ dysfunction syndrome(MODS).Methods The clinical data of 53 cases with pulmonary tuberculosis complicated by MODS were retrospectively analyzed.Univariate and multivariate analysis were used to examine the screened risk factors associated with pulmonary tuberculosis complicated by MODS.Results Univariate analysis showed that APACHE Ⅱ scores,extent of lung lesion,level of serum albumin and...

Analysis on the clinical characteristics of 100 cases of elderly pulmonary tuberculosis complicated by fungal infection

GAO Xu-Sheng, YU Xue-Yan, GUO Shuai, 吕Yan

Chinese Journal of Antituberculosis. 2010, 32(10):  58-62. 

Abstract ( 1352 )   PDF (212KB) ( 493 )   Save

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Objective To explore the epidemic condition,risk factors,clinical characteristics,diagnosis and treatment in elderly patients with pulmonary tuberculosis complicatied by fungal infection.Methods 100 paitents with elderly pulmonary tuberculosis complicated by fungal infection hospitalized from 2006 to 2010 in Shandong Chest Hospital were analyzed retrospectively.Results Most of the cases were retreated patients who had a long course of disease,extensive lesions,broad-spectrum antibiotic used and immunosuppre...

Management and evaluation of Free Anti-TB Drug Supply System in Shaanxi Province

XIAO Hui-Ling, ZHANG Tian-Hua, LIU Chun-Yan

Chinese Journal of Antituberculosis. 2010, 32(10):  63-66. 

Abstract ( 1355 )   PDF (180KB) ( 372 )   Save

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Objective To evaluate the current situation of free anti-TB drug supply system in order to improve its performance.Methods Drug supply system was established simultaneously with DOTS strategy.Monitoring and evaluation of this system was done by on-site supervision,quarterly monitoring.Results From 2002 to 2004,Shaanxi province set up a complete three-level free anti-TB drugs supply system.The system worked well,and ensured free treatment of nearly 70 thousands pulmonary tuberculosis patients through uninter...

The floating population of tuberculosis patients in touch-type collapse of the District management study of evaluation

ZHANG Xing-Shu, YANG Kun-Yun, DENG Xue-Hui, WU Zi-Jian

Chinese Journal of Antituberculosis. 2010, 32(10):  68-70. 

Abstract ( 1289 )   PDF (155KB) ( 339 )   Save

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Objective Through the floating population of tuberculosis patients in the management of the implementation of the collapsed area touch-type studies,toexplore a set of scientific and practical and easy tooperate long-term management mechanisms and implementation of the touch-type,and sciebtific evaluation of management effectiveness.Methods Choose from January of 2008 to December of 2009 patient.s relativey more HuNan province XangXi and Shao Yang at floating population pulmonary tuberculosis of ShenZhen.The...