Abstract: Objective To compare the effect of different Mycobacterium tuberculosis antigens on enzyme-linked immunospot assay(ELISPOT).Methods 132 tuberculosis patients and 90 control cases were recruited to the research.The peripheral blood mononuclear cells(PBMC) of all participants were co-cultured with the following antigens: purified protein derivative(PPD),early secretory antigenic target-6(ESAT-6) and ESAT-6/culture filtrate protein-10 fusion protein(ESAT-6/CFP-10,E/C).Spot forming cells(SFC) were enumerated by...

Key words:  tuberculosis/diagnosis, antigens bacterial, bacterial protein, recombinant fusion proteins, immunoenzyme techniques