The Clinical Value of Detecting Tb-DNA in BALF by FQ-PCR in Diagnosing Pulmonary Tuberculosis

Abstract

Abstract: Objective To study the value of detecting M.tuberculosis DNA in bronchoalveolar lavage fluid(BALF) by fluorescent quantitative polymerase chain reaction(FQ-PCR) in diagnosing pulmonary tuberculosis.Methods The BALF from the 187 patients with pulmonary tuberculosis treated first and 41 patients with pneumonia were detected the M.tuberculosis DNA(TB-DNA) by FQ-PCR,and performed mycobacterial culture.The bronchoscopic brushing smears were detected by acid-fast staining.Results The positive rates of detecting B...

Key words: tuberculosis, pulmonary/diagnosis, bronchoalveolar lavage fluid, DNA, bacterial, polymerase chain reaction

CLC Number:

R440

LI Zhi-Hui, ZHANG Hong-Man, LIU Jian-Qiang, LIU Peng-Chong, ZHAO Jie, LI Yi, CUI Dan, FENG Xiu-Li. The Clinical Value of Detecting Tb-DNA in BALF by FQ-PCR in Diagnosing Pulmonary Tuberculosis[J]. Chinese Journal of Antituberculosis, 2010, 32(10): 32-34.

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The Clinical Value of Detecting Tb-DNA in BALF by FQ-PCR in Diagnosing Pulmonary Tuberculosis

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