Abstract: Objective To express,purify Mycobacterium tuberculosis(M.tb) Rv2352c protein in E.coli and study its antigenicity.Methods The M.tb rv2352c gene was amplified by PCR and then cloned into the expression plasmid pET30a(+).The positive clones were analyzed and transformed into E.coli BL21(DE3).The bacteria expressing Rv2352c were cultured in different concentration of IPTG.The fusion protein was purified by Ni+-NTA column,detected by SDS-PAGE,and reacted with the sera from tuberculosis patients by Western blot....

Key words: Mycobacterium tuberculosis, bacterial proteins, antigens, bacterial, recombinant fusion proteins