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Chinese Journal of Antituberculosis ›› 2010, Vol. 32 ›› Issue (10): 20-24.

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Research on mycobacteria culture and drug sensitivity assay with microscopic observation method

Liang Bowen; Lu Yu; Chu Naihui   

  1. Department of Pharmacology; Beijing Tuberculosis and Thoracic Tumor Research Institute; Beijing 101149; China;
  • Online:2010-10-15 Published:2011-08-25

Abstract: Objective To assess the value of microscopic observation method(MOM) on mycobacteria culture and drug sensitivity assay.Methods The smear-positive sputum specimens were cultured and directly determined the drug-susceptibility test on isoniazid(INH),rifampicin(RFP),streptomycin(SM) and ethambutol(EMB)with MOM,compared with conventional culture on Lǒwenstein-Jensen(L-J) medium and drug susceptibility testing using the absolute concentration method.The inconsistent results were further detected with DNA suquen...

Key words: Mycobacterium tuberculosis, microbiol sensitivity tests, microscopy

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