Chinese Journal of Antituberculosis ›› 2020, Vol. 42 ›› Issue (2): 101-107.doi: 10.3969/j.issn.1000-6621.2020.02.005
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SUN Jin-xia,ZHANG Qing-wen,LI Yin-hong,JIANG Xin()
Received:
2019-11-13
Online:
2020-02-10
Published:
2020-02-19
Contact:
Xin JIANG
E-mail:jiangxingao@163.com
SUN Jin-xia,ZHANG Qing-wen,LI Yin-hong,JIANG Xin. Study of autophagy induced by myricetin in MTB infected macrophages through PI3K/Akt/mTOR signaling pathway[J]. Chinese Journal of Antituberculosis, 2020, 42(2): 101-107. doi: 10.3969/j.issn.1000-6621.2020.02.005
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杨梅素 (μmol/L) | 24h细胞存活率 (%, | t值a | P值a | 48h细胞存活率 (%, | t值b | P值b | 72h细胞存活率 (%, | t值c | P值c |
---|---|---|---|---|---|---|---|---|---|
空白组 | 99.35±0.76 | 101.90±1.28 | 94.50±2.85 | ||||||
12.5 | 96.99±3.45 | 0.67 | 0.54 | 92.13±1.82 | 5.34 | 0.01 | 89.75±3.36 | 1.08 | 0.34 |
25 | 90.68±0.44 | 9.95 | 0.00 | 92.07±2.00 | 4.13 | 0.02 | 89.54±1.49 | 1.54 | 0.20 |
50 | 88.39±1.26 | 7.45 | 0.00 | 91.57±1.53 | 5.18 | 0.01 | 91.24±0.96 | 1.09 | 0.34 |
100 | 92.40±0.68 | 6.83 | 0.00 | 93.82±0.47 | 5.93 | 0.00 | 86.48±2.26 | 2.21 | 0.09 |
200 | 89.03±0.86 | 9.02 | 0.00 | 92.13±1.82 | 4.38 | 0.01 | 79.65±1.36 | 4.71 | 0.01 |
组别 | LC3Ⅱ蛋白相对灰度值 ( | t值 | P值 | p62蛋白相对灰度值 ( | t值 | P值 |
---|---|---|---|---|---|---|
空白组 | 0.36±0.01 | 0.51±0.02 | ||||
模型组 | 0.52±0.01 | 8.85a | 0.00a | 0.86±0.02 | 10.98c | 0.00c |
杨梅素12.5μmol/L组 | 0.59±0.02 | 2.97b | 0.04b | 0.72±0.01 | 6.50d | 0.00d |
杨梅素25μmol/L组 | 0.65±0.01 | 7.91b | 0.00b | 0.85±0.00 | 0.81d | 0.46d |
杨梅素50μmol/L组 | 0.71±0.01 | 9.77b | 0.00b | 0.60±0.02 | 9.53d | 0.00d |
杨梅素100μmol/L组 | 0.83±0.01 | 16.37b | 0.00b | 0.58±0.01 | 12.01d | 0.00d |
组别 | p-Akt蛋白相对灰度值 ( | t值 | P值 | p-mTOR蛋白相对灰度值 ( | t值 | P值 |
---|---|---|---|---|---|---|
空白组 | 0.19±0.01 | 0.27±0.00 | ||||
模型组30min | 1.23±0.01 | 84.60a | 0.00a | 0.28±0.01 | 0.60f | 0.58f |
模型组60min | 1.52±0.01 | 66.79a | 0.00a | 0.27±0.01 | 0.36f | 0.74f |
模型组180min | 0.74±0.02 | 30.49a | 0.00a | 0.57±0.00 | 94.61f | 0.00f |
空白+杨梅素 | 0.19±0.00 | 0.92b | 0.41b | 0.29±0.00 | 0.91g | 0.41g |
杨梅素30min | 0.99±0.01 | 27.60c | 0.00c | 0.29±0.01 | 1.31h | 0.26h |
杨梅素60min | 0.96±0.01 | 30.06d | 0.00d | 0.33±0.01 | 2.31i | 0.08i |
杨梅素180min | 0.43±0.01 | 18.60e | 0.00e | 0.46±0.01 | 21.60j | 0.00j |
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