新疆维吾尔族人群HLADR、DQ基因多态性与结核病易感性的研究

中国防痨杂志 ›› 2011, Vol. 33 ›› Issue (4): 197-203.

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新疆维吾尔族人群HLADR、DQ基因多态性与结核病易感性的研究

王喜¹ 任玲君¹ 李秀玲¹ 吴芳² 章乐² 张万江²

1.河南开封市疾病预防控制中心,开封,475000; 2.石河子大学新疆地方与民族高发病教育部重点实验室,石河子,832002

收稿日期:2011-08-06 出版日期:2011-04-20 发布日期:2012-03-16
通信作者: 张万江 E-mail:zwj1117@sina.com
基金资助:
国家自然科学基金资助(30760237)

A study on the correlation between the polymorphism of HLA-DR、DQ genes and the susceptibility to pulmonary tuberculosis in a Uyghur population from Xinjiang

Wang Xi¹, Ren Lingjun¹, Li Xiuling¹, Wu Fang², Zhang Le², Zhang Wanjiang²

1.Kaifeng Center Disease of Control and Prevention; Kaifeng Henan 475000; 2.Key Laboratory of Xinjiang Endemic and Ethnic Disease; Shihezi University; Shihezi Xinjiang 832002; China

Received:2011-08-06 Online:2011-04-20 Published:2012-03-16
Contact: Zhang Wanjiang E-mail:zwj1117@sina.com

摘要/Abstract

摘要： 目的探讨人类白细胞抗原(HLA)-DRB1、DQB1基因多态性与新疆维吾尔族人群结核病易感性的关联。方法采用病例-对照的研究方法，应用聚合酶链反应-序列特异性引物(PCR-SSP）技术对226例新疆维吾尔族肺结核病患者(肺结核病例组)和231例新疆维吾尔族健康对照者(健康对照组)进行HLA-DRB1、DQB1基因分型，比较其等位基因频率(GF)，并计算其比值比(OR)。结果 1. 肺结核病例组中HLA-DRB1*11基因频率显著高于健康对照组，2组的GF分别为4.5%、1.1%，差异有统计学意义(OR=4.388，95%CI=1.618～11.905，P_c＜0.05)；肺结核病例组中HLA-DRB1*04基因频率显著高于健康对照组，2组的GF分别为12.8%、8.4%，但P值经过校正后差异无统计学意义(OR=1.686，95%CI=1.060～2.684，P_c>0.05)。 2. 肺结核病例组中HLA-DQB1*0201基因频率显著高于健康对照组，2组的GF分别为40.1%、19.2%，差异有统计学意义(OR=3.379，95%CI=2.302～4.960，P_c＜0.05)；肺结核病例组中HLA-DQB1*0301/4基因频率显著低于健康对照组，2组的GF分别为6.2%、10.3%，但P值经过校正后差异无统计学意义(OR=0.561、95%CI=0.334～0.941，P_c＞0.05)。结论 HLA-DRB1*11、DQB1*0201等位基因与新疆维吾尔族人群结核病强相关，DRB1*11、DQB1*0201可能是其易感基因。

关键词: 结核, 肺, HLA-DR抗原, HLA-DQ抗原, 多态现象, 遗传, 疾病遗传易感性, 新疆［维吾尔自治区］

Abstract: Objective To explore the correlation between the polymorphism of HLA-DRB1、DQB1 genes and the susceptibility to pulmonary tuberculosis (PTB) in a Uyghur population from Xinjiang. Methods Using case-control study, HLA-DRB1、DQB1 genotypes in 226 PTB cases and 231 healthy controls from Uyghur population in Xinjiang were analyzed with polymerase chain reaction-sequence specific primers (PCR-SSP), in which gene frequency (GF) and odds ratio (OR) were calculated and compared. Results 1.The frequency of HLA-DRB1*11 gene in PTB cases was significantly higher than that in the healthy controls (OR=4.388, 95%CI=1.618—11.905, P_c＜0.05), their GFs were 4.5% and 1.1% respectively. The frequency of HLA-DRB1*04 gene in PTB cases was higher than that in the healthy controls, but there was the statistical difference (OR=1.686, 95%CI=1.060—2.684, P_c>0.05), their GFs were 12.8% and 8.4% respectively. 2. The frequency of HLA-DQB1*0201 gene in PTB cases was significantly higher than that in the healthy controls (OR=3.379, 95%CI=2.302—4.960, P_c＜0.05), their GFs were 40.1% and 19.2% respectively. The HLA-DQB1*0301/4 gene occurred less frequently in TB patients than in controls, but there was the statistical difference (OR=0.561, 95%CI=0.334—0.941, P_c＞0.05), their GFs were 6.2% and 10.3% respectively.  Conclusion HLA-DRB1*11 and DQB1*0201 alleles were evidently correlated to the incidence of tuberculosis, maybe susceptible genes of tuberculosis in Xinjiang Uyghur population.

Key words: tuberculosis,pulmonary, HLA-DR antigens, HLA-DQ antigens, polymorphism,genetic, genetic predisposition to disease, Xinjiang

王喜任玲君李秀玲吴芳章乐张万江. 新疆维吾尔族人群HLADR、DQ基因多态性与结核病易感性的研究[J]. 中国防痨杂志, 2011, 33(4): 197-203.

Wang Xi,Ren Lingjun,Li Xiuling,Wu Fang,Zhang Le,Zhang Wanjiang. A study on the correlation between the polymorphism of HLA-DR、DQ genes and the susceptibility to pulmonary tuberculosis in a Uyghur population from Xinjiang[J]. Chinese Journal of Antituberculosis, 2011, 33(4): 197-203.

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新疆维吾尔族人群HLADR、DQ基因多态性与结核病易感性的研究

A study on the correlation between the polymorphism of HLA-DR、DQ genes and the susceptibility to pulmonary tuberculosis in a Uyghur population from Xinjiang

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