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中国防痨杂志 ›› 2011, Vol. 33 ›› Issue (6): 372-376.

• 论著 • 上一篇    下一篇

痰标本采集、储存及运输过程对培养结果影响的分析

宋红焕1 孟尔旺2 时金艳2 彭红1 许卫国1   

  1. 1.江苏省疾病预防控制中心; 2.江苏省连云港第四人民医院
  • 收稿日期:2011-04-08 出版日期:2011-06-20 发布日期:2012-03-16
  • 通信作者: 许卫国 E-mail:jsjkmck@163.com

Analysis of influencing factors for culture results during sputum collection,storage and transport

 Song Honghuan1; Meng Erwang2; Shi Jinyan2; Peng Hong1; Xu Weiguo1   

  1.  1.Jiangsu Center for Disease Control and Prevention; Nanjing 210009; China; 2.the Fourth Hospital of Lianyungang; Lianyungang 222000; China
  • Received:2011-04-08 Online:2011-06-20 Published:2012-03-16
  • Contact: Xu Weiguo E-mail:jsjkmck@163.com

摘要: 目的 通过分析某市级结核病实验室痰培养数据,总结县级运送阳性痰标本至市级进行痰培养工作模式对痰培养结果的影响因素。 方法 对江苏省某市级结核病实验室2010年4月—10月痰培养数据进行分析。 结果 该实验室初诊涂阳病人痰培养共计475例,对痰标本采集、储存、运输、培养等各个环节进行分析,得出:不同送样县区(P=0.007),不同痰标本数量(P=0.008),不同送样时间间隔(P=0.037),不同痰标本阳性级别(P=0.001)的痰培养结果均有统计学意义。 结论 由县级运送痰标本至市级进行痰培养,应在采集标本时严格按照《中国结核病防治规划实施工作指南》(以下简称《指南》)要求采集即夜晨3份痰标本,提高送检痰标本质量,尽快送检,并加强对不同县区样品采集运送人员的培训。

关键词: 分枝杆菌, 结核, 痰, 细菌学技术

Abstract: Objective To analyze the influencing factors for sputum culture during sputum collection, storage and transport.  Methods The data of sputum culture from a municipal TB lab in Jiangsu province from April to October in 2010 were analyzed.  Results The process including sputum specimen collection, storage, transportation and culture in 475 smear-positive patients were analyzed, and found that the sputum specimens from different counties (P=0.007), different specimen quantity (P=0.008), different specimen storage time(P=0.037)and different positive class (P=0.001)all had statistic significance for culture quality.  Conclusions The procedure of specimen collection, storage, transportation and culture influence the result of culture. Their standard procedure required by the NTP should be strictly followed, and the training for health workers in county laboratory should be strengthened to improve sputum culture quality.

Key words: Mycobacterium tuberculosis, sputum, bacteriological techniques