结核病患者血清中Small RNAs表达谱分析

中国防痨杂志 ›› 2011, Vol. 33 ›› Issue (6): 350-356.

结核病患者血清中Small RNAs表达谱分析

钟球周琳钱明陈涛陈亮李海成

广东省结核病防治研究所,广州,510630

收稿日期:2011-03-28 出版日期:2011-06-20 发布日期:2012-03-16
通信作者: 钟球 E-mail:gdtb_bg@vip.163.com
基金资助:
广东省自然科学基金(9151051501000003); 国家十一五重大专项:结核病发病模式的研究项目(2008ZX10003-007)

Analysis of the expression profile for serum small RNAs from tuberculosis patients

Zhong Qiu,Zhou Lin,Qian Ming,Chen Tao,Chen Liang,Li Haicheng

Guangdong Institute for Tuberculosis Prevention and Treatment; Guangzhou 510630; China

Received:2011-03-28 Online:2011-06-20 Published:2012-03-16
Contact: Zhong Qiu E-mail:gdtb_bg@vip.163.com

摘要/Abstract

摘要： 目的了解结核病患者血清中Small RNA(sRNA)表达谱的特征，为从Small RNA角度探讨结核病发生、发展的调控机制奠定基础。方法采用Solexa深度测序技术得到包括miRNA、siRNA、piRNA、rRNA、tRNA、snRNA、snoRNA、repeat associate sRNA、exon或intron降解片段等在内的所有Small RNA。通过与已知数据库进行比对、寻找样品与数据库之间在基因组位置上的overlap等方法，对sRNA进行注释，同时选取没有被注释上的sRNA，使用华大自主开发的软件Mireap预测novel miRNA。结果非结核病组血清中发现109 630种、2 759 795条Small RNA，其中候选miRNA种数为84、候选miRNA总表达量为1 433；结核病组血清中发现97 614种、1 452 119条Small RNA，其中候选miRNA种数为65、候选miRNA总表达量为755。结论结核病人与非结核病人血清中的Small RNA表达谱存在种类与数量上的差异。

关键词: 结核/血液, 微RNAs, 基因表达

Abstract: Objective To analyze the expression profile of serum small RNAs (sRNA) from tuberculosis (TB) patients, and find a new way to study the regulatory mechanisms in the process of tuberculosis. Methods Small RNAs from Solexa deep sequencing covered almost every kind of RNA, including miRNA, siRNA, piRNA, rRNA, tRNA, snRNA, snoRNA，repeat associated sRNA and degraded tags of exon or intron. By comparing our sequences with those in databases and picking out the overlap on genome location between our data and the databases, sRNAs can be annotated into different categories. Those which can not be annotated will be used to predict novel miRNA by the self-developed software Mireap. Results In the sera from non-TB patients group, 109 630 different types containing 2 759 795 sequences of small RNAs were found,in which there were 84 different types containing 1 433 sequences of miRNA candidates. In the sera from TB patients group, 97 614 different types containing 1 452 119 sequences of small RNAs were found, in which there were 65 different types containing 755 sequences of miRNA candidates. Conclusion The expression profiles of serum small RNAs are different between TB patients and non-TB patients.

Key words: tuberculosis/blood, micro RNAs, gene expression

钟球周琳钱明陈涛陈亮李海成. 结核病患者血清中Small RNAs表达谱分析[J]. 中国防痨杂志, 2011, 33(6): 350-356.

Zhong Qiu,Zhou Lin,Qian Ming,Chen Tao,Chen Liang,Li Haicheng. Analysis of the expression profile for serum small RNAs from tuberculosis patients[J]. Chinese Journal of Antituberculosis, 2011, 33(6): 350-356.

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