Table of Content

10 April 1999, Volume 21 Issue 4

Previous Issue    Next Issue

Probing the analysis methods on project coverage and case_finding data of M O H TB control project

Wang Lixia,Duanmu Hongjin,Song Wenhu,et al.

. 1999, 21(4):  181-184. 

Abstract ( 1166 )   PDF (255KB) ( 346 )   Save

References | Related Articles | Metrics

Objective To probe the analysis methods on project coverage and case_finding data in order to directed against the untidy development of M O H TB control project at the beginning period.Method Considering the character of project trundling development,we determine the registration level of smear positive cases on cycle bases.And the indicator of project coverage level was developed in order to reflect the multiple development level of the project.Result and Conclusion The results showed that the two indicators could reflect the overall level of the project coverage and case_finding and be comparable among different project counties.The uneven development of the project was showed up very protrusive through analysis.The authors analysised the reasons of uneven development.

The sample survey on drug resistant tuberculosis in Henan,China,1996

Wang Guobin,Peng Yili, Zhang Guolong,et al.

. 1999, 21(4):  185-190. 

Abstract ( 1229 )   PDF (418KB) ( 401 )   Save

References | Related Articles | Metrics

Objective From April to December 1996,Henan province as first member in China to join WHO global Drug Resistance Surveillance(DRS)network to implement DRS at the 30 tuberculosis dispensaries selected by cluster sampling method in order to reference data for TB control.Method According to WHO DRS guideline,randomly selected 30 counties,newly registered smear positive TB cases were enrolled in order and rechecked their treatment history.Through Z_N fast acid stain,L_J medium culture,proportion method for drug sensitivity test,drug susceptibility testing against streptomycin(SM),isoniazid(INH),rifampicin(RIF),and ethambutol(EMB)four drugs were performed.Laboratory quality control were done by Korean SRL,All the data analyzed by WHO SDRTB software and χ 2 statistical test. Results 1.1372 TB cases were enrolled project,916 new cases and 456 previously treated cases,2.Primary resistance(PR)to any drug was found 43.0%,Acquired resistance(AR)to any drug was 68.2%,total resistance rate 51.4%. 3. Any primary resistance SM,32.5%;INH,31 0%;RIF,20.7% and EMB,10.3%.and any acquired resistance SM,52.2%;INH,49.3%;RIF,48 3%;and EMB,20.4%. 4.Multi_drug resistance rate primary 16.5%,Acquired 37.1%. 5.PR and PMDR in never use drug group is 35.0% and 10.9%,far less than that in using drugs<1 month group 66.1%and 25.3%( P<0.01).6. >60 years group,PR and AR is 39.1% and 58.4% far less than average rate 43.0% and 68.3%.7.The cure rate of new cases all sensitive group,mono_drug resistance and multi_drug resistance was 64.3%,59.6%,49.8% and of old cases,50.0%,37.7%,35.8%.Conclusion Drug resistant TB was found highly prevalent in Henan and the cure rate remained poor.The finding urges to take immediate action to improve cure rate.

Experimental study on coronary artery lesion in rabbit with acute tuberculous infection

Yang Qi,Lu Runsheng,Jiang Shaoshuang,et al.

. 1999, 21(4):  191-193. 

Abstract ( 1161 )   PDF (258KB) ( 328 )   Save

References | Related Articles | Metrics

Objective To observe the coronary artery lesion that is led by Mycobacterium tuberculosis.Method Japanese rabbit were research infection animals.H 37 Rv was the research bacterium strain.One group rabbits were injected tuberculous solution,after Co60 damaging immune system function,another group rabbite were directly injected tuberculous solution.We observed the lesions of heart,specially the coronary artery by pathological method,including ZN dyeing,HE dyeing,three colour dyeig(MCT),BCG immunehistochemisty dyeing and electron microscope examination.Result The result show that Mycobacterium tuberculosis can to any place of heart by blood and form lesion.Severe damage was found in coronary artery.Immunization suppression rabbits will be resulted in susceptility to Mycobacterium tuberculosis and cause severe lesions.Conclusion Mycobacterium tuberculosis can cause the lesion of coronary artery.

DNA fixation technique on influence of hybridization results of PCR products of M.tuberculosis

Li Chuanyou,Ma Yu,Xing Aiying,et al.

. 1999, 21(4):  194-195. 

Abstract ( 1150 )   PDF (140KB) ( 287 )   Save

References | Related Articles | Metrics

Objective To compare influence of various DNA fixation methods on hybridization of PCR products of M.tuberculosis.Methods PCR products of M.tuberculosis were obtained,then diluted into different concentration and added to the nylon membrane.PCR products on the nylon membrane were fixed with UV crosslinking,oven baking and alkali fixation method,then hybridized and analysed.Results It was found that DNA fixation method had a great influence on detection of PCR products of M.tuberculosis.Alkali fixation had a good sensitivity and reproducibility.Conclusion All the results suggested that PCR laboratory of M.tuberculosis could consider using alkali fixation to PCR products,then hybridized and detected.

Use of MTT for rapid detection of BCG viability influence of different anti_mycobacterial drugs

Lu Bin,Xu Shunqing,Chen Zhifei.

. 1999, 21(4):  196-200. 

Abstract ( 1188 )   PDF (303KB) ( 412 )   Save

References | Related Articles | Metrics

Objective To use the ability of viable cells to reduce 34,5dimethylthiazol2yi2,5diphenyltetrazolium bromide(MTT)to set up a test to detect 4 antimycobacterial drugs'influence on viability of invitro cultured BCG.Method To detect different concentration of 4 antimycobacterial drugs—Rifampin,Streptomycin,Isoniazid and Paminosalicylic acid's viability influent level in BCG.Result The assay shows a linear relationship between the number of vibable bacteria and the ability to reduce MTT.BCG exposed to antimycobacterial drugs showed concentrationdependent inhibition of the ability to reduce MTT.The inhibition paralleled the reduction in the number of colony forming units(CFU).Conclusion The assay can be visually read and requires less than 3 days to obtain susceptibility results.It is a cheap and rapid screening method for BCG vitality and be possible a cheap and rapid screening method for multidrugresistant M.tuberculosis.

Clinical analysis of pulmonary tuberculosis complicating with moraxella catarrhalis infection

Deng Lihong,Liu Dongyang,Tian Xizhong,et al.

. 1999, 21(4):  201-202. 

Abstract ( 1370 )   PDF (140KB) ( 260 )   Save

References | Related Articles | Metrics

Objective 58 case of pulmonary tuberculosis complicating with moraxella catarhalis were analyzed to demontrate the characteristics of this infection.Method We use Doerm creteria to quantify the bacteria in sputum,three times consecutively.Result Pulmonary tubercuiosis is easily complicated by the bacteria.The clinic and X_ray manifestation were short of feature,and only 43% with high blood cell count.Conclusion Pulmonary tuberculosis patients with unexplained fever,purulent sputum and newly_appeared moist rales in the lung,moraxella becteria infection should be alert of,β_lactamase inhibitors must recommended to control the infection.

A study on chemotherapy and drug resistance for 122 cases of relapse pulmonary tuberculosis

Zhao Li,Zhao Ting.

. 1999, 21(4):  203-205. 

Abstract ( 1225 )   PDF (228KB) ( 304 )   Save

References | Related Articles | Metrics

bjective To analyse the antituberculosis_drug resistance and chemotherapy of sputum culture_positive in 122 cases with recurrent pulmonary tuberculosis(RPT).Methods Drug_susceptibility tests was done in sputum culture_positive cases with RPT between 1995 and 1997.HREZ/HRE was used as therapeutic scheme.Sputum examination for tubercle bacillus was performed three times at least.Results The sputum negative conversion rates at the end of the chemotherapy in the drug_susceptible group(91 cases)and the drug_resistant group(31 cases)were 85.7% and 67.7%,respectively.Conclusions If the drug_resistance,particularly for multidrug_resistant,occurred in recurrent cases during retreatment,the therapeutic efficacy is less satisfactory than that in the drug_susceptible cases.It is important to control the infectious source of new treated cases actively and reduce occurrence of drug_resistant cases for tuberculosis control project.

Observation of the effect on liver function in pulmonary tuberculosis with positive HBVM treated with rifapentine or rifampicin

Li Dongfang.

. 1999, 21(4):  206-207. 

Abstract ( 1400 )   PDF (143KB) ( 313 )   Save

References | Related Articles | Metrics

Objective To evaluate the effect on liver function in pulmonary tuberculosis with positive HBVM treated with rifapentine or rifampicin.Method In HBVM positive group and negative group treated with rifapentine or rifampicin,liver function injury were observed before and after treatment.Results The incidence of liver injury in treated rifampicin group is higher than that in treated rifapentine group( P<0.01).The incidence of liver injury in HBVM positive group is higher than that in HBVM negative group( P<0 01).In treated rifampicin group,the incidence of liver injury in HBVM positive group is higher than that in HBVM negative group( P<0.01).In treated rifapentine group showed liver injury no significant differences( P >0 05).Conclusion The incidence of liver injury in HBVM positive group is higher than that in HBVM negative group because liver injury exists in the former before treatment.In the treatment of pulmonary tuberculosis,using rifapentine is better and safer.

Analysis of effect of draining sputum by different drug ultrasonic nebulization to check tuberculosis

Zhang Aiqin,Wang Guobin,Zhen Xinan,et al.

. 1999, 21(4):  208-209. 

Abstract ( 1358 )   PDF (132KB) ( 261 )   Save

References | Related Articles | Metrics

Objective Check the tuberculosis in patients who are nonsputum pulmonary cases,improve the sputum checking rate.Method 362 outpatients sampled randomly in three groups,use(1)3.6% salt water(2)0.1% peppermint water(3)10% salt water respectively to check the TB bacilli by ultrasonic nebulization.Results The draining sputum rate of the second group( P<0.01)is obviousy higher than the first and the third.The sputum positive rate of the first group(22.1%)is obviously higher than the second group(6.8%) and is nearly the same with the third (14.2%).There is no difference in the effetive sputum positive sputum checking rate( P >0.05)among the three groups.Conclusion 3.6% salt water can both drain sputum from the 73.1% of the nonsputum patients and check tuberculosis from the 22.1% of the nonsputum patients,moreover,it's convenient and economical and can be used widely.

Clinical analysis of 74 cases of Heroin user with pulmonary tuberculosis in Xinjiang

Jie Li,Partamu,Amenguli.

. 1999, 21(4):  210-212. 

Abstract ( 1349 )   PDF (316KB) ( 398 )   Save

References | Related Articles | Metrics

Objective To explore the clinical characteristics of pulmonary tuberculosis cases depending on Heroin.Method The general condition,clinical manifestation and therapeutic changes of 74 hospitalized cases of heroin user with pulmonary tuberculosis were systematic analyzed.Result All the group of cases are youngsters (17～32 years old),Their condition are serious,more complication and poor compliance.There were 7cases died in the period of hospitalization.Conclusion In view of the tuberculosis epidemic situation on narcotic person,we must pay attention to this matter and resolve progressively.

Evaluation of the clinical application of two kinds of L J medium

Zhang Li,Yang Litao,Li Aihua,et al.

. 1999, 21(4):  213-214. 

Abstract ( 1674 )   PDF (125KB) ( 313 )   Save

References | Related Articles | Metrics

Objective To analyze the availability of no starch L J medium recommended by WHO.Methods The separation rate,growth speed and drug sensitivity test of M tuberculosis were observed to compare L J medium with improved L J medium.Results The primitive separation rate of M tuberculosis cultivated in the two mediums didn't show obvious statistic difference( P >0.05),and the growth speed were similar.The total relativity rate of the drug sensitivity test was 97 3%.Conclusion It may not be necessary to add potato starch in the compound of improved L J medium.

Study on the centralized management model of tuberculosis

Zhu Kuangji.

. 1999, 21(4):  215-217. 

Abstract ( 1245 )   PDF (220KB) ( 670 )   Save

References | Related Articles | Metrics

Objective In order to enhance the course of TB control project in our county and to research centralized management model.Methods All cases were carried out by the management model of one step(direct)centralization in combination with two step(indirect)centralization.For internal,for example,city special hospital and basic clinic were carried out two step centralization.For external,e g the out department of TB preventive centre of our station was carried out one step centralization.Results The smear positive new register rate in 1998 is 26.94/100?000,increased by 4.7 times more than the average level of forward three years,increased by 2.6 times from that in 1997.Conclusion Proceed from the actual situation.Take measures suited to local conditions.It is also an available management model which is to carried out one step centralization in combination with two step contralization of TB centralized management model.

Analysis of cost and benefit about conducting DOTS management to the patients with pulmonary tuberculosis

Gao Xiaoping.

. 1999, 21(4):  218-220. 

Abstract ( 1087 )   PDF (209KB) ( 515 )   Save

References | Related Articles | Metrics

Clinical analysis of 12 cases of pulmonary tuberculosis with pulmonary candidiasis

Li Jirui,Song Taili,Fang Guoying.

. 1999, 21(4):  221-222. 

Abstract ( 1250 )   PDF (157KB) ( 290 )   Save

References | Related Articles | Metrics

Objective To discuss decreasing occurence and misdiagnosis of pulmonary tuberculosis with pulmonary candidiasis.Methods 12 cases were diagnosed pulmonary candidiasis.Based on:1.The patients presented fever,cough or primary symptom exaggerated.2.X ray examining showed new lesions or primary lesions enlarged.3.Same fungus grew on the culture for three times continuously.4.After antimycosis treatment,the patients condition got well,symptom dismissed,fungus didn't grow on the culture of the sputum.The above material were studied retrospectively.Results Their sphere of lesions of pulmonary tuberculosis were large,There are many cavities,high positive rate of mycobacteria(8/12).high fever,cough,expectoration,leukocytosis(15～35×10.9/L)and used antibacterial?corticoid drugs in these patients.Most of them were misdiagnosed.Conclusion Candidiasis were misdiagnosed easily and must be paid attention.It should be decreased for using regularly antibacterial?corticoid drugs.