肺结核患者外周血CD4CDhigh25 T细胞的临床意义初探

摘要/Abstract

摘要： 目的通过测定肺结核患者外周血CD4CD^high₂₅T细胞的表达，探讨CD4CD^high₂₅T细胞的的临床意义。方法 2008年1月至2010年2月选取住院患者及门诊做体检的健康志愿者共78例，其中肺结核患者40例，门诊健康志愿者38例，应用流式细胞技术测定外周血T细胞表面分子CD4、CD8、CD4CD^high₂₅，以百分比表示表面分子阳性T细胞占淋巴细胞的比例。结果外周血CD4CD^high₂₅T细胞，肺结核组（1.9%±1.3%）明显高于健康组（0.7%±1.0%），差异有统计学意义（t=5.237, P<0.01）。外周血CD4T细胞，肺结核组（47.9%±11.2%）低于健康组（56.0%±14.6%），差异有统计学意义（t=2.830, P<0.01）。外周血CD8T细胞，肺结核组（26.2%±6.1%）低于健康组（33.0%±11.1%），差异无统计学意义（t=0.353, P>0.05）。肺结核患者治疗2月后，CD4CD^high₂₅T细胞（1.8%±0.8%）有下降，但与治疗前比较差异无统计学意义（t=0.811，P>0.05）。CD4T细胞、CD8T细胞均有升高（51.6%±14.4%，33.7%±11.5%），但差异无统计学意义（t值分别为=1.496, 0.570，均P>0.05）。结论 CD4CD^high₂₅T细胞参与了肺结核患者的细胞免疫过程，发挥抑制CD4和CD8T细胞的活化与增殖的作用，从而影响了患者的临床演变及预后。

关键词: 结核, 肺/血液, T淋巴细胞, 流式细胞术

Abstract: Objective To observe theclinical significance of CD4CD^high₂₅T cells from peripheral blood in pulmonary tuberculosis (PTB) patients.  Methods From January 2008 to February 2010, 40 hospitalized PTB patients and 38 healthy controls from outpatient department for physical examination were enrolled. CD4, CD8 and CD4CD^high₂₅T cells which were presented by their percentages in peripheral blood lymphocytes of the above subjects were assessed by flow cytometry.  Results The frequencies of CD4CD^high₂₅T cells in PTB patients （1.9%±1.3%）were significantly higher than that in healthy controls （0.7%±1.0%）(t=5.237, P<0.01). The frequencies of CD4T cells in PTB patients （47.9%±11.2%）were significantly lower than that in healthy controls （56.0%±14.6%）(t=2.830, P<0.01). The frequencies of CD8T cells in PTB patients（26.2%±6.1%） were somewhat lower than that in healthy controls （33.0%±11.1%）, but there was not statistical difference（t=0.353, P>0.05）. After 2 months of anti-TB treatment, the frequencies of CD4CD^high₂₅T cells in PTB patients （1.8%±0.8%）were decreased as compared with pre-treatment but without statistic significance (t=0.811, P>0.05), while the frequencies of CD4T cells and CD8T cells in PTB patients （51.6%±14.4%，33.7%±11.5%）were all increased as compared with pre-treatment but without statistic significance (t=1.496, 0.570, respectively, all P>0.05).  Conclusions CD4CD^high₂₅T cells are involved in cellular immune of PTB patients, inhibiting the activation and proliferation of CD4 and CD8T cells, affecting the clinical manifestations and prognosis of PTB.

Key words: tuberculosis,pulmonary/blood, T-lymphocytes, flow cytometry

张青肖和平粟波. 肺结核患者外周血CD4CD^high₂₅ T细胞的临床意义初探[J]. 中国防痨杂志, 2011, 33(2): 95-98.

Zhang Qing,Xiao Heping,Su Bo. Clinical significance of CD4CD^high₂₅ T cells in patients with pulmonary tuberculosis[J]. Chinese Journal of Antituberculosis, 2011, 33(2): 95-98.

参考文献

［1］Lazarevic V, Dawn N, Flynn JL. Long-term control ofMycobacterium tuberculosisinfection is mediated by dynamic immune responses［J］.J Immunol, 2005,175(2):1107-1117.
［2］ Fontenot JD, Rasmussen JP, Williams LM, Dooley JL, Farr AG, Rudensky AY. Regulatory T cell lineage specification by the fork head transcription factor foxp3［J］. Immunity, 2005,22(3):329-341.
［3］ Belkaid Y, Rouse BT. Natural regulatory T cells in infectious disease［J］. Nat Immunol, 2005,6(4):353-360.
［4］ Pandiyan P, Zheng L, Ishihara S, Reed J, Lenardo MJ. CD4CD25＋Foxp3＋regulatory T cells induce cytokine deprivation-mediated apoptosis of effector CD4T cells［J］. Nat Immunol, 2007,8(12):1353-1362.
［5］ Chiacchio T, Casetti R, Butera O, Vanini V, Carrara S, Girardi E, Di Mitri D, Battistini L, Martini F, Borsellino G, Goletti D. Characterization of regulatory T cells identified as CD4(+)CD25(high)CD39(+) in patients with active tuberculosis［J］. Clin Exp Immunol, 2009,156(3):463-470.
［6］ Mahan CS, Thomas JJ, Boom WH, Rojas RE. CD4CD25(high) Foxp3+ regulatory T cells down regulate human Vdelta2+ T-lymphocyte function triggered by anti-CD3 or phosphoantigen［J］. Immunology, 2009, 127(3):398-407.
［7］ Li L, Lao SH, Wu CY. Increased frequency of CD4(+)CD25(high) Treg cells inhibit BCG-specific induction of IFN-gamma by CD4(+) T cells from TB patients［J］. Tuberculosis, 2007,87(6):526-534.
［8］中华医学会.临床诊疗手册(结核病分册)［M］.北京:人民出版社,2005:80.
［9］ American Thoracic Society. Targeted tuberculin testing and treatment of latent tuberculosis infection. Am J Respir Crit Care Med, 2000,161(suppl):S221-S247.
［10］Derrick SC, Evering TH. Characterization of the protective T-cell response generated in CD4-deficient mice by a live attenuatedMycobacterium tuberculosisvaccine［J］. Immunology, 2007,120(2):192-206.
［11］ Walrath J, Zukowski L. Resident Th1-like effector memory cells in pulmonary recall responses toMycobacterium tuberculosis ［J］. Am J Respir Cell Mol Biol, 2005,33(1):48-55.
［12］ Piccirillo CA, Shevach EM. Naturally-occurring CD4CD25+ immunoregulatory T cells: central players in the arena of peripheral tolerance［J］. Semin Immunol, 2004,16(2):81-88.
［13］ Suffia IJ, Reckling SK, Piccirillo CA, Goldszmid RS, Belkaid Y. Infected site-restricted Foxp3+ natural regulatory T cells are specific for microbial antigens［J］. J Exp Med, 2006,203(3):777-788.
［14］ Lellem A, Mariani M, Lang R, Recalde H, Panina-Bordignon P, Siningaglia F, D’Ambrosio D. Unique chemotactic response profile and specific expression of chemokine receptors CCR4 and CCR8 by CD4CD25+regulatory T cells［J］. J Exp Med, 2001,194(6):847-885.
［15］ Jean-Michel Hougardy, Virginie Verscheurea, Camille Locht, Francoise Mascart. In vitro expansion of CD4tCD_high²⁵FOXP3+CD127low/-regulatory T cells from peripheral blood lymphocytes of healthyMycobacterium tuberculosis -infected humans［J］.Microbes Infect, 2007,9(11): 1325-1332.
［16］Chen X, Zhou B, Li M, Deng Q, Wu X, Le X, Wu C, Larmonier N, Zhang W, Zhang H, Wang H, Katsanis E.CD4(+)CD25(+)FoxP3(+) regulatory T cells suppressMycobacterium tuberculosisimmunity in patients with active disease［J］. Clin Immunol, 2007,123(1):50-59.

[1]	刘晓莉, 雷丽梅, 郭周莉, 黄殷, 徐静, 赵霞, 王燕, 付莉. 结核病患者产生病耻感与领悟社会支持的相关性研究[J]. 中国防痨杂志, 2020, 42(9): 1002-1008.
[2]	中国防痨协会学术工作委员会《中国防痨杂志》编辑委员会. 抗结核药品固定剂量复合制剂的临床使用专家共识[J]. 中国防痨杂志, 2020, 42(9): 885-893.
[3]	靳鸿建. 我国县级结核病防治服务体系建设及防治工作亟需加强——一位老防痨工作者的意见和建议[J]. 中国防痨杂志, 2020, 42(9): 896-902.
[4]	张灿有, 夏辉, 成君. Ⅱ级生物安全柜在结核病实验室中的检测及报告要求[J]. 中国防痨杂志, 2020, 42(9): 903-909.
[5]	周林, 刘二勇, 孟庆琳, 陈明亭, 周新华, 高微微, 林明贵, 谢汝明. 《WS 288—2017 肺结核诊断》标准实施后肺结核诊断质量评估分析[J]. 中国防痨杂志, 2020, 42(9): 910-915.
[6]	刘二勇, 王前, 周林, 张国钦, 张修磊, 马永成, 杨枢敏, 王毳, 孟庆琳, 陈明亭, 林明贵, 屠德华. 我国部分地区病原学检测阴性肺结核诊断质量现状分析[J]. 中国防痨杂志, 2020, 42(9): 916-920.
[7]	孟庆琳, 李进岚, 林定文, 马永成, 侯双翼, 刘年强, 周林. 结核病防治从业人员对新的结核病标准相关知识知晓情况调查分析[J]. 中国防痨杂志, 2020, 42(9): 921-925.
[8]	王前, 周林, 刘二勇, 赵雁林, 李涛, 陈明亭, 杨丽佳, 王嘉. 我国县级结核病定点医疗机构结核病诊断能力现况调查研究[J]. 中国防痨杂志, 2020, 42(9): 926-930.
[9]	李婷, 何金戈, 苏茜, 李京, 李运葵, 高文凤, 高媛, 杨文. 结核菌素试验在四川省布拖县HIV感染/AIDS患者中筛查结核感染的价值[J]. 中国防痨杂志, 2020, 42(9): 931-936.
[10]	李运葵, 何金戈, 苏茜, 李婷, 李京, 高文凤, 杨文, 毛光玉. 结核病症状筛查在四川省布拖县HIV感染/AIDS患者中发现结核病患者的价值[J]. 中国防痨杂志, 2020, 42(9): 937-941.
[11]	苏茜, 夏勇, 逯嘉, 王丹霞, 何金戈. 2009—2018年四川省0~14 岁儿童肺结核流行特征分析[J]. 中国防痨杂志, 2020, 42(9): 942-947.
[12]	邓亚丽, 张天华, 刘卫平, 张宏伟, 马煜, 李鹏. 2014—2018年陕西省肺结核发病的时空聚集性分析[J]. 中国防痨杂志, 2020, 42(9): 948-955.
[13]	董晓, 赵珍, 刘年强, 王森路, 崔燕. 2009—2017年新疆维吾尔自治区老年肺结核发现特征分析[J]. 中国防痨杂志, 2020, 42(9): 956-961.
[14]	马廷龙, 韩毅, 程序, 刘志东. 超声抗结核药品电导入联合化疗对胸壁结核的疗效观察[J]. 中国防痨杂志, 2020, 42(9): 968-972.
[15]	南海, 张芸, 杨新婷, 段鸿飞. GeneXpert MTB/RIF对骨关节结核诊断价值的Meta分析[J]. 中国防痨杂志, 2020, 42(9): 973-980.

肺结核患者外周血CD4CD^high₂₅ T细胞的临床意义初探

Clinical significance of CD4CD^high₂₅ T cells in patients with pulmonary tuberculosis

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

友情链接