关键词: 分枝杆菌, 结核, 利福平, 抗药性, 细菌, 核酸扩增技术, 敏感性与特异性

Abstract: Objective  To evaluate the performance of Xpert Mtb/RIF test on detecting tuberculosis and rifampin resistance for patients.   Methods  Recruited 588 initial diagnosis patients, selected sputum samples to do smear, solid culture, traditional proportion method drug susceptibility test and Xpert Mtb/RIF test. Analyze the sensitivity and specificity of Xpert Mtb/RIF method in detecting tuberculosis and refampin resistance.  Results  According to the solid culture result, the sensitivity and specificity of Xpert Mtb/RIF test in detecting tuberculosis are 95.0% (345/363) and 89.6% (147/164). According to the conventional drug susceptibility test result, the sensitivity and specificity in detecting rifampin resistance are 89.1% (41/46) and 96.3% (289/300) respectively.  Conclusion  The Xpert Mtb/RIF test has very high sensitivity and specificity. It has very good application prospects in rapidly detection tuberculosis and rifampin resistance.

Key words: Mycobacterium tuberculosis, Rifampin, Drug resistance, bacterial, Nucleic acid amplification techniques, Sensitivity and specificity