甘露聚糖结合凝集素基因A/B位点与新疆维吾尔族结核病的相关性研究

中国防痨杂志 ›› 2012, Vol. 34 ›› Issue (7): 445-451.

甘露聚糖结合凝集素基因A/B位点与新疆维吾尔族结核病的相关性研究

周江张万江

832002 石河子大学新疆地方与民族高发病教育部重点实验室［周江（研究生，现在 432000 湖北省孝感市中心医院超声科工作）、张万江］

收稿日期:2011-11-07 出版日期:2012-07-10 发布日期:2012-07-06
通信作者: 张万江 E-mail:zwj1117@sina.com
基金资助:
国家自然科学基金 (30960355)

Correlation between MBL-A/B gene with the susceptibility of tuberculosis in Xinjiang Uighur population

ZHOU Jiang，ZHANG Wan-jiang

Department of Pathophysiology, Medical School, Shihezi University/Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University, Shihezi 832002, China ［Postgraduation，Now worked at Ultrasound Department of Hubei Xiaogan Central Hospital,Xiaogan 432000,China］

Received:2011-11-07 Online:2012-07-10 Published:2012-07-06
Contact: ZHANG Wan-jiang E-mail:zwj1117@sina.com

摘要/Abstract

摘要： 目的探讨甘露聚糖结合凝集素（mannan-binding lectin，MBL）基因的多态性是否与新疆维吾尔族人群结核病发病相关。方法通过使用引物序列特异性PCR(SSP-PCR)的方法分别对226例新疆维吾尔族活动性肺结核患者（简称病例组）及231例有结核分枝杆菌接触史的新疆同民族健康者（简称对照组）进行MBL基因的A/B多态性位点进行基因分型，统计学分析采用病例对照分析研究不同基因型与新疆维吾尔族结核病易感性的关系。结果在病例组中，MBL基因A/B位点 AA 基因型106例(占46.90%)，AB基因型106例（占46.90%)，BB基因型14例(占6.20%)；对照组AA基因型则为146例(占63.20%)，AB基因型80例(占34.63%)，BB基因型5例(占2.17%)。病例组中MBL-AB突变基因频率显著高于健康对照组，两组的突变基因频率分别为6.20%、2.17%，差异有统计学意义(χ²=5.224，P_c＜0.05)。结论新疆维吾尔族人群中MBL基因AA基因型可能为结核病的保护性因素，BB基因型可能为结核病发病的危险性因素。新疆维吾尔族人群中MBL基因A/B位点多态性与结核病易感性有明显相关性。

关键词: 结核,肺, 分枝杆菌,结核, 甘露糖结合外源凝集素, 聚合酶链反应, 疾病遗传易感性, 新疆［维吾尔自治区］

Abstract: Objective To study the relationship between the polymorphism of the MBL gene and the susceptibility of tuberculosis (TB) in Xinjiang Uighur population. Methods The polymorphism of the MBL-A/B gene was analyzed by PCR-sequence specific primer (SSP) in 226 patients with pulmonary TB and 231 healthy controls who had a history of exposure to M. tuberculosis in Xinjiang Uighur population. The relationship between MBLgenotypes and the susceptibility of pulmonary TB was studied in accordance to the case-control study, and cases were grouped according to the genotypes. Results In the case group, AA, AB and BB genotypes at MBL gene A/B site were 106 cases (46.90%), 106 cases (46.90%) and 14 cases (6.20%), respectively. In the control group, AA, AB and BB genotypes were 146 cases (63.20%), 80 cases (34.63%) and 5 cases (2.17%), respectively. The frequency of BB genotypes at MBL gene A/B site in the case group was significantly higher than that in the healthy control group (6.20% vs 2.17%)(χ²=5.224，P_c＜0.05). Conclusion AA genotype of MBL gene may be protective factor for TB, while BB genotype may be risk factor for TB in Xinjiang Uighur population.

Key words: Tuberculosis,pulmonary, Mycobacterium tuberculosis, Mannosebinding lectin, Polymerase chain reaction, Genetic predisposition to disease, Xinjiang

周江张万江. 甘露聚糖结合凝集素基因A/B位点与新疆维吾尔族结核病的相关性研究[J]. 中国防痨杂志, 2012, 34(7): 445-451.

ZHOU Jiang，ZHANG Wan-jiang. Correlation between MBL-A/B gene with the susceptibility of tuberculosis in Xinjiang Uighur population[J]. Chinese Journal of Antituberculosis, 2012, 34(7): 445-451.

参考文献

［1］李萍,贾天军,石万元,等. 北方汉、回、蒙三民族健康人群MBL54,52,57位密码子基因多态性.广东医学, 2008, 29(1)：57-59.

［2］中华人民共和国卫生部.卫生部介绍全国肺结核疫情状况现状［EB/OL］.(2011-03-21)［2011-05-13］.http：//www.moh.gov.cn.

［3］金鑫，范新春，吴卫东.新疆维吾尔自治区第四次结核病流行病抽样调查报告.中国防痨杂志，2004，26（5）：254-267.

［4］Bo1dt AB， Petzl-Erler ML. A new strategy for mannose-binding lectin gene haplotyping.Hum Mutat,2002,19(3):296-306.

［5］Ledwina T，Gnot S. Testing for Hardy-Weinberg equilibrium. Biometrics, 1980,36(1):161-165.

［6］王建华. 流行病学.北京：人民卫生出版社，2008：66-67.

［7］钟成全，余新沛，王方勇,等.中国维吾尔族人群MBL基因CGT52TGT.GGC54GAC和GGA57GAA点突变基因频率的研究.南方医科大学学报，2006,26（12）：1764-1767.

［8］Madsen HO, Garred P, Thiel S, et al. Interplay between promoter and structural gene variants control basal serum level of mannan-binding protein.J Immunol,1995，155（6）:3013-3020.

［9］Madsen HO, Satz ML, Hogh B, et al. Different molecular events result in low protein levels of mannan-binding lectin in populations from southeast Africa and South America.J Immunol，1998，161（6）:3169-3175.

［10］Steffensen R, Thiel S,Varming K, et al. Detection of structural gene mutations and promoter polymorphisms in the mannan-binding lectin (MBL)gene by polymerase chain reaction with sequence-specific primers.J Immunol Methods, 2000，241（1/2）:33-42.

［11］Matsushita M, Miyakawa H, Tanaka A, et al.Single nucleotide polymorphisms of the mannose-binding lectin are associated with susceptibility to primary biliary cirrhosis. J Autoimmun, 2001，17（3）:251-257.

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