支气管灌洗液抑癌基因过甲基化对肺癌诊断价值的研究

摘要/Abstract

摘要： 目的探讨支气管灌洗液抑癌基因过甲基化对肺癌诊断的应用价值。方法选取北京市结核病胸部肿瘤研究所收治怀疑肺癌患者78例，包括肺癌51例和良性病变患者27例，从支气管灌洗液上清和细胞提取DNA，对上清游离DNA进行定量分析，并采用NMSP完成对支气管灌洗液上清和细胞的过甲基化分析，所选择的靶基因包括p16，MGMT以及RASSF1A基因。将NMSP结果与临床诊断比较，计算敏感性和特异性。结果中心型肺癌、周围型肺癌和肺部良性病变支气管灌洗液上清游离DNA含量没有显著差异。支气管灌洗液细胞抑癌基因过甲基化诊断中心型肺癌的敏感性和特异性分别为85.2%和81.5%，诊断周围型肺癌的敏感性和特异性分别为54.2%和81.5%。支气管灌洗液上清和细胞抑癌基因过甲基化诊断中心型肺癌的敏感性和特异性为88.9%和70.4%，诊断周围型肺癌的敏感性和特异性为75.0%和70.4%。结论支气管灌洗液抑癌基因的过甲基化可为肺癌诊断提供帮助。

关键词: 肺肿瘤, 支气管肺泡灌洗液, O(6)-甲基鸟嘌呤DNA甲基转移酶, 肿瘤抑制蛋白质类, 甲基化

Abstract: Objective To evaluate the value of anti-oncogene hypermethylation in bronchial lavages fluid （BLAF） for the diagnosis of primary lung cancer. MethodsSeventy-eight patients with lung tumors (containing 51 patients with lung cancer and 27 patients with benign pulmonary lesions) from Beijing Tuberculosis and Thoracic Tumor Research Institute were selected. DNAs from supernate and cell in bronchial lavages were extracted, quantified, and detected promoter hypermethylation of three target genes, including p16, MGMT and RASSF1A. Using final clinical diagnosis as control, the sensitivity and specificity of promoter hypermethylation in the diagnosis of lung cancer were analyzed. ResultsDNA concentrations from supernate of bronchial lavages had not significant difference among central lung cancer, peripheral lung cancer and pulmonary benign lesions group. The sensitivity and specificity of anti-oncogene hypermethylation from the bronchial lavages cells for the diagnosis of central lung cancer were 85.2% and 81.5% respectively; for peripheral lung cancer were 54.2% and 81.5%, respectively. Combining with the detection of bronchial lavages supernates, the sensitivity and specificity for the diagnosis of central lung cancer were 88.9% and 70.4% respectively, for peripheral lung cancer were 75.0% and 70.4% respectively.ConclusionsHypermethylation of anti-oncogene might be an useful marker for the diagnosis of lung cancer.

Key words: lung neoplasms, Bronchoalveolar lavage fluid, O(6)-Methylguanine-DNA methyltransferase, tumor suppressor proterins, methylation

段鸿飞,傅瑜,李琦,赖百塘,汪慧,岳文涛,张洪涛,王小珏,马玙. 支气管灌洗液抑癌基因过甲基化对肺癌诊断价值的研究[J]. 中国防痨杂志, 2009, 31(5): 277-283.

Duan Hongfei, Fu Yu, Li Qi,Lai Baitang,Wang Hui,Yue Wentao,Zhang Hongtao,Wang Xiaojue,Ma Yu. Study on anti-oncogene hypermethylation in bronchial lavages fluid for the diagnosis of primary lung cancer[J]. Chinese Journal of Antituberculosis, 2009, 31(5): 277-283.

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