关键词: 偶然分支杆菌, 聚合酶链反应, DNA探针, 鉴定

Abstract: Objective To determine the pathogen of the post-injection infection outbreak in Nanping at gene level with molecular biological technique and to establish a rapid identification method of M.fortuitum by PCR/RFLP and DNA probe technique.Methods A single pair of prime and oligonucleotide probe of M.fortuitum were designed,according to the sequence of mycobacterium of 16S-23S rDNA spacer sequences.29 clinical strains were amplified by PCR,then dot-blot hybridization and RFLP of PCR products were made.Results 470bp fragment were produced by PCR of 29 clinical strains,three fragment were produced by Hae Ⅲ,MSP Ⅰ digest of PCR products respectively.The probe only hybridized with M.fortuium and 29 clinical strains.Conclusion The Results showed that the pathogen of infection outbreak were M.fortuitum.The 16S-23S rDNA PCR investigative system is sensitive and specific,which can identify M.fortuitum at gene level.

Key words: M.fortuitum, PCR, DNA hybridization, Identification