关键词: 结核,肺, 分枝杆菌,结核, 血浆, 蛋白质组学, 生物学标记

Abstract:

Objective To study the plasma proteomics of specific protein markers in patients with secondary pulmonary tuberculosis, to explore specific protein markers of secondary pulmonary tuberculosis. Methods Blood samples were obtained from 30 secondary pulmonary tuberculosis (tuberculosis group) and 30 healthy controls and a certain protein mass spectrometry was detected using label-free quantitative technology. If the protein abundance of the tuberculosis group more than 2 (up-regulated) or <0.5 (down-regulated) compared with that of the control group, and P<0.05 using t test, it was considered to be a differential protein bioinformatics analysis of differential proteins was performed using GO and KEGG. The difference in the average protein abundance between the two groups was analyzed by t test and P<0.05 was considered statistically significant. Results A total of 518 differential proteins were found in the two groups. Of them, 256 proteins were up-regulated and 262 were down-regulated. Bioinformatics analysis showed that the main functions of differential proteins were binding (29.79%, 406/1363) and protein binding (24.80%, 338/1363), and cell process (14.75%, 467/3167) and metabolic process (11.11%, 352/3167) were the main processes biological they involved.By cell location analysis, it was found that most of the proteins were located within the cell (22.07%, 389/1763) and cell (22.01%, 388/1763). KEGG analysis showed that 17 up-regulated pathways (such as dorsal ventral axis pathway and focal adhesion pathway) and 1 down regulated pathway were different between tuberculosis group and control group. Conclusion The plasma protein of patients with secondary pulmonary tuberculosis may have changed significantly, which provides valuable clues for exploring the mechanism of the secondary pulmonary tuberculosis.

Key words: Tuberculosis,pulmonary, Mycobacterium tuberculosis, Plasma, Proteomics, Biological markers