天津市监狱羁押人员中结核病患者临床分离菌株的基因型分析

doi:10.3969/j.issn.1000-6621.2019.03.022

摘要/Abstract

摘要：

为揭示结核分枝杆菌(MTB)北京基因型菌株中基于差异区域(RD)多态性形成的各个进化分支在天津监狱羁押人员中的流行情况,笔者收集2013年1月至2016年6月从天津市监狱羁押人员结核病患者痰标本中分离培养获得的124株MTB菌株进行基因型分析。结果发现,124株菌株中经鉴定为北京基因型者有116株(93.5%)。116株北京基因型菌株中,96株(82.8%)为北京基因型现代株,20株(17.2%)为北京基因型古代株。基于大片段多态性分析,北京基因型MTB分为4个进化分支。20株北京基因型古代株中有7株(6.0%)为RD181(+)菌株,13株(11.2%)为RD181(-)菌株;96株北京基因型现代株中,95株(81.9%)为RD150(+)菌株,仅1株(0.9%)为RD150(-)菌株。北京基因型MTB的各个进化分支中,RD150(+)北京基因型现代株在监狱中传播流行最为广泛。结果提示需加强RD150(+)北京基因型现代株在天津监狱羁押人员中流行的监测工作。

关键词: 分枝杆菌, 结核, 基因测定, 基因型, 分子流行病学, 囚犯

Abstract:

The aim of study was to evaluate the prevalence of the sublineages of Mycobacterium tuberculosis (MTB) Beijing genotype circulating strains based on the region of differences polymorphisms in the prisons in Tianjin, China. Overall 124 MTB strains, which were isolated from the sputum samples of patients with pulmonary tuberculosis in the prisons in Tianjin from Jan. 2013 to Jun. 2016, were included. Beijing genotype strains accounted for 93.5% (116/124). Among these Beijing genotype strains, 96 (82.8%) were modern Beijing strains, and 20 (17.2%) were ancient Beijing strains. Based on large fragment polymorphism analysis, the 116 Beijing genotype strains were further grouped in four sublineages. Among the 20 ancient Beijing strains, 7 (6.0%) strains were RD181(+), and 13 (11.2%) strains were RD181(-). Of the 96 modern Beijing strains, 95 belonged to RD150(+) sublineage, accounting for 81.9% of the Beijing genotype stains, and only 1 (0.9%) strain with RD150 deletion was found in this study. RD150(+) modern Beijing sublineage were predominant among the prisoners in Tianjin. Our data showed the need for continued surveillance of RD150(+) modern Beijing sublineage transmission among prison inmates in Tianjin, China.

Key words: Mycobacterium tuberculosis, Genetic testing, Genotype, Molecular epidemiology, Prisoners

谢彤,王春花,赵慧,巨韩芳,穆成,王志锐,孙蕊. 天津市监狱羁押人员中结核病患者临床分离菌株的基因型分析[J]. 中国防痨杂志, 2019, 41(3): 361-363. doi: 10.3969/j.issn.1000-6621.2019.03.022

Tong XIE,Chun-hua WANG,Hui ZHAO,Han-fang JU,Cheng MU,Zhi-rui WANG,Rui SUN. Genotypic analysis of Mycobacterium tuberculosis among prison population in Tianjin, China[J]. Chinese Journal of Antituberculosis, 2019, 41(3): 361-363. doi: 10.3969/j.issn.1000-6621.2019.03.022

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北京基因型分支	差异序列			NTF区IS6110	菌株数 (株)	构成比 (%)
北京基因型分支	RD181	RD150	RD142	NTF区IS6110	菌株数 (株)	构成比 (%)
1	+	+	+	-	7	6.0
2	-	+	+	-	13	11.2
3	-	+	+	+	95	81.9
4	-	-	+	+	1	0.9