Abstract: Objective To evaluate the prevalence of HIV/Mycobacteria coinfection with liquid Mycobacteria culture and molecular technique in North China.  Methods Fifty-three suspected HIV/AIDS patients were performed with liquid culture and strain identification of Mycobacteria from 2009 to 2011. The clinical manifestation，radiological imaging and relevant laboratory data from all patients with definite diagnosis of HIV/Mycobacteria coinfection were analyzed.  Results Of 19 patients confirmed with HIV/Mycobacteria coinfection, Mycobacteriosis involved in pulmonary only in 11(57.9%),extra-pulmonary (brain and liver) in 2(10.5%), Mycobacteremia in 4(21.1%)，both pulmonary and mycobacteremia in 2(10.5%). Mycobacterium tuberculosis(16,84.2%) and Mycobacterium avium-intracellulare complex (3,15.7%)were isolated from the 19 patients. 15(78.9%) patients had a quite low CD4⁺T lymphocytes count less than 100 cells per microlitre in peripheral blood.  Conclusion Combining liquid culture and molecular techniques is effective method to identify HIV/Mycobacteria coinfection and to be applied in the clinical practice.

Key words: Superinfection, HIV infections, Mycobacterium infections, Mycobacterium, Bacteriological techniques, Culture media