2017年绍兴地区106株非结核分枝杆菌菌种鉴定结果分析

doi:10.3969/j.issn.1000-6621.2019.11.007

摘要/Abstract

摘要：

目的分析绍兴地区非结核分枝杆菌(NTM)的流行情况,为NTM诊疗提供科学依据。方法对绍兴地区2017年临床分离的972株分枝杆菌,采用结核分枝杆菌抗原检测胶体金法进行结核分枝杆菌复合群和NTM的初步菌种鉴定,结果106株为NTM,占分枝杆菌培养阳性菌株的10.91%(106/972),对初步鉴定为NTM的菌株应用 DNA微阵列芯片法进行NTM菌种鉴定。结果 106株NTM菌株经DNA微阵列芯片法鉴定,共有14个菌种,其中菌株数量前3位的菌种为:胞内分枝杆菌[55株(51.89%)]、鸟分枝杆菌[19株(17.92%)]、堪萨斯分枝杆菌[13株(12.26%)]。其余分别为:龟-脓肿分枝杆菌[4株(3.77%)]、瘰疬分枝杆菌[3株(2.83%)]、戈登分枝杆菌[3株(2.83%)]、土分枝杆菌[2株(1.89%)]、其他[7株(6.60%)]。结论 2017 年绍兴地区NTM 临床分离率占分枝杆菌培养阳性菌株的10.91%;NTM种类多,以胞内分枝杆菌、鸟分枝杆菌、堪萨斯分枝杆菌为主要流行菌种。

关键词: 分枝杆菌,非典型性, 细菌分型技术, 芯片分析技术, 小地区分析

Abstract:

Objective Analyze the prevalence of non-tuberculous mycobacteria (NTM) in Shaoxing and provide a scientific basis for NTM diagnosis and treatment.Methods In the Shaoxing area, 972 strains of mycobacteria were isolated in 2017, and the preliminary strain identification of Mycobacterium tuberculosis complex and NTM was carried out by using the colloidal gold method of Mycobacterium tuberculosis antigen detection. 106 strains were NTM, accounting for mycobacterial culture-positive strains 10.91% (106/972), for the initial identification of NTM strains using DNA microarray method for NTM strain identification.Results One hundred and six strains of NTM were identified by DNA microarray method. There were 14 species of bacteria. The top 3 strains were 55 strains of Mycobacteria intracellulare (51.89%), 19 strains of Mycobacterium avium (17.92%) and 13 strains of Mycobacterium kansasii (12.26%). The remaining ones were: 4 strains of Mycobacteria chelonae-abscessus (3.77%), 3 strains of Mycobacterium scrofulaceum (2.83%), 3 strains of Mycobacterium gordonae (2.83%), and 2 strains of Mycobacterium terrae (1.89%), the other 7 strains (6.60%).Conclusion In 2017, the clinical isolation rate of NTM in Shaoxing accounted for 10.91% of the positive strains of mycobacterial culture, and there were many NTM species. Mycobacterium intracellulare, Mycobacterium avium and Mycobacterium kansasii were the main epidemic strains of NTM in Shaoxing.

Key words: Mycobacteria, atypical, Bacterial typing techniques, Microchip analytical procedures, Small-area analysis

金法祥,陶学芳,王华钧,许文芳,陈雪芳. 2017年绍兴地区106株非结核分枝杆菌菌种鉴定结果分析[J]. 中国防痨杂志, 2019, 41(11): 1179-1183. doi: 10.3969/j.issn.1000-6621.2019.11.007

JIN Fa-xiang,TAO Xue-fang,WANG Hua-jun,XU Wen-fang,CHEN Xue-fang. Analysis of identification results of 106 strains of non-tuberculous mycobacteria in Shaoxing area in 2017[J]. Chinese Journal of Antituberculosis, 2019, 41(11): 1179-1183. doi: 10.3969/j.issn.1000-6621.2019.11.007

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菌种名称	株数	构成比 (%)
胞内分枝杆菌(M.intracellulare)	55	51.89
鸟分枝杆菌(M.avium)	19	17.92
堪萨斯分枝杆菌(M.kansasii)	13	12.26
龟-脓肿分枝杆菌(M.chelonae-abscessus)	4	3.77
瘰疬分枝杆菌(M.scrofulaceum)	3	2.83
戈登分枝杆菌(M.gordonae)	3	2.83
土分枝杆菌(M.terrae)	2	1.89
偶然分枝杆菌(M.fortuitum)	1	0.94
不产色分枝杆菌(M.nonchromogenicum)	1	0.94
金色分枝杆菌(M.aurum)	1	0.94
海-溃疡分枝杆菌(M.marinum-ulcerans)	1	0.94
蟾蜍分枝杆菌(M.xenopi)	1	0.94
浅黄分枝杆菌(M.gilvum)	1	0.94
苏尔加-玛尔摩分枝杆菌(M.szulgai-malmoense)	1	0.94