IL-18启动子区-137G/C和-607C/A位点多态性与结核病易感性关系——Meta分析

摘要/Abstract

摘要： 目的应用Meta分析探讨IL-18基因启动子区单核苷酸多态性（single nucleotide polymorphism，SNP）等位基因位点-137和 -607与结核病易感性的关系。方法收集采用序列特异性引物聚合酶链反应（PCR-SSP）及测序技术检测SNP位点-137和 -607的研究，在严格的质量评价基础上，通过统计学方法应用Review Manager 5.0软件进行了Meta分析。入选文献标准：(1)关于IL-18启动子区SNP -137 G/C和(或)-607 C/A与结核易感性关联的病例-对照研究；（2）对照组基因分布符合Hardy-Weinberg（H-W）遗传平衡定律；（3）原始资料完整；（4）文献中提供基因型频率或等位基因频率；（5）纳入文献语言为中文或英文；（6）多态性检测方法通过PCR-SSP及测序技术或SNaPshot SNP分型方法。文献排除标准：（1）重复报告的研究；（2）数据不清或等位基因不清的文献；（3）基于家系的研究；（4）对照组基因分布没有进行H-W遗传平衡检验的研究。结果 IL-18启动子区SNP -607 C/A位点研究6项，患者695例，正常对照879例；SNP -137 G/C位点研究5项，患者594例，正常对照781例。结核病组IL-18启动子区等位基因-137 G和 -607 C与对照组相比，等位基因-137 C和 -607 A的合并OR值分别为1.41（95% CI为1.15～1.74，P=0.001）和0.93（95% CI为0.81～1.08，P=0.36）。结论 IL-18启动子区SNP位点-137 G/C与结核病易感性相关联，提示IL-18启动子SNP（-137）是结核病的易感位点之一; SNP位点-607 C/A与结核病易感性之间关联无统计学意义。

关键词: 结核, 白细胞介素18, 多态性, 单核苷酸, Meta分析

Abstract: Objective To provide a quantitative summary in estimating the association between the polymorphisms of two loci in interleukin-18 (IL-18) gene promoter and susceptibility of tuberculosis by Meta-analysis. Methods The polymorphisms of IL-18 promoter gene -137 G/C and -607 C/A were analyzed using the sequence specificity primer polymerase chain reaction (PCR-SSP) and gene sequencing analysis. Based on the strict quality evaluation, statistics was carried out for Meta analysis by Review Manager 5.0 software. Inclusion criteria were as follow: (1) the studies on the association between the polymorphisms of IL-18 promoter gene -137 G/C and -607 C/A and the tuberculosis susceptibility; (2) the gene distribution of the control group coincides to the law of genetic equilibrium of HardyWeinberg; (3) the original material was complete; (4) the study provided the genotype frequency or allele frequency; (5) the study was published in Chinese or English; (6) the polymorphisms were detected by PCR-SSP and gene sequencing or SNaPshot SNP technology. Exclusions criteria were as follow: (1) the repeated report; (2) the data were not clear; (3) the study was based on the family; (4) the gene distribution of the control group was not inspected by the law of H-W genetic equilibrium. Results According the established inclusion criteria，6 studies with a total of 695 cases and 879 controls were identified in the SNP -607 C/A of the promoter of IL-18 gene. The SNP -137 G/C was identified in 5 studies which contained 594 cases and 781 controls. The pooled odds ratios (ORs) for studies with -137 C and -607 A loci allele variants in the IL-18 promoter gene were 1.41（95% CI: 1.15～1.74，P=0.001）and 0.93（95% CI为0.81～1.08，P=0.36），respectively，compared with -137 G and -607 C alleles．Conclusion SNP -137G/C located in IL-18 promoter was associated with tuberculosis, and may be one of the susceptibility loci of tuberculosis. The association of SNP -607 C/A and tuberculosis susceptibility showed no statistically significant difference in our study.

Key words: Tuberculosis, Interleukin-18, Polymorphism,single necleotide, Meta analysis

张喆马红霞于学文王秋萍杨健郭佑民. IL-18启动子区-137G/C和-607C/A位点多态性与结核病易感性关系——Meta分析[J]. 中国防痨杂志, 2011, 33(12): 815-819.

ZHANG Zhe, MA Hong-xia, YU Xue-wen, WANG Qiu-ping, YANG Jian, GUO You-min. Association between the polymorphisms of interleukin-18 promoter gene -137 G/C and -607 C/A and the susceptibility of tuberculosis: a Meta-analysis[J]. Chinese Journal of Antituberculosis, 2011, 33(12): 815-819.

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