康乐霉素A体外抗结核活性的初步研究

中国防痨杂志 ›› 2007, Vol. 29 ›› Issue (2): 160-162.

康乐霉素A体外抗结核活性的初步研究

高凌玉¹；张健源²；李传友²；周建琴¹；游雪甫¹；王振³；汪月¹；孙承航¹；

1中国医学科学院中国协和医科大学医药生物技术研究所北京 100050；2北京市结核病胸部肿瘤研究所北京 101149；3桂林医学院药学院桂林 541004；

出版日期:2007-02-10 发布日期:2007-11-03
基金资助:
科研院所社会公益研究专项(项目编号:2005DIB1J090);;国家“十五”重大研究专项(项目编号:2004AA2Z3784);;中国医学科学院,中国协和医科大学医药生物技术研究所研究启动基金

Preliminary studies on inhibitory activity of Kanglemycin A,against Mycobacterium tuberculosis

Gao Linyu,Zhang Jianyuan,Li Chuanyou,et al.

Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100050,China

Online:2007-02-10 Published:2007-11-03

摘要/Abstract

摘要： 目的发现新安莎类抗生素,康乐霉素A体外抗结核活性。方法应用色谱技术从地中海诺卡氏菌康乐变株1747-64发酵液中分离康乐霉素A,并采用试管二倍稀释法,进行康乐霉素A对耻垢分枝杆菌（ATCC14468）,结核分枝杆菌H₃₇Rv（ATCC27294）,H₃₇Ra（ATCC25177）,临床分离的氧氟沙星（OFLX）敏感结核分枝杆菌以及临床分离的耐氧氟沙星结核分枝杆菌最低抑制浓度（Minimal Inhibitor Concentration,MIC）的试验。结果康乐霉素A对耻垢分枝杆菌活性弱,MIC为64μg/ml,对结核分枝杆菌H₃₇Rv,H₃₇Ra的MIC为8μg/ml,对临床分离的氧氟沙星敏感结核分枝杆菌菌株的MIC范围为18μg/ml,临床分离的耐氧氟沙星结核分枝杆菌菌株的MIC范围为116μg/ml。结论新安莎类抗生素,康乐霉素A体外具有明显的抗结核活性,有望成为新抗结核药物或先导化合物。

关键词: 康乐霉素A, 分枝杆菌结核, 最低抑菌浓度

Abstract: Objective To explore the bioactivities of Kanglemycin A,a novel ansamycin group antibiotic against M.tuberculosis(TB)in vitro. Methods Kanglemycin A was purified by silica gel chromatography and sephadex LH-20 chromatography,and its MICs to 20 strains including M.smegmatis,M.tuberculosis H₃₇Rv,M.tuberculosis H₃₇Ra,ofloxacin(OFLX)-sensitive M.tuberculosis clinical isolates and OFLX-resistant M.tuberculosis isolates were determined by equivalent serial dilution method. Results Kanglemycin A MIC to M.smegmatis was 64ug/ml,to M.tuberculosis H₃₇Rv and H₃₇Ra was 8ug/ml,to OFLX-sensitive M.tuberculosis isolates were 1-8?ug/ml,to OFLX-resistant M.tuberculosis isolates were 1-16ug/ml. Conclusion Kanglemycin A had strong inhibitory activity against M.tuberculosis,and might be a potential anti-TB drug candidate or leading compound.

Key words: Kanglemycin A, Mycobacterium tuberculosis, MIC

高凌玉,张健源,李传友,周建琴,游雪甫,王振,汪月,孙承航,. 康乐霉素A体外抗结核活性的初步研究[J]. 中国防痨杂志, 2007, 29(2): 160-162.

Gao Linyu,Zhang Jianyuan,Li Chuanyou,et al.. Preliminary studies on inhibitory activity of Kanglemycin A,against Mycobacterium tuberculosis[J]. Chinese Journal of Antituberculosis, 2007, 29(2): 160-162.

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