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中国防痨杂志 ›› 2010, Vol. 32 ›› Issue (12): 818-821.

• 论著 • 上一篇    下一篇

FQ-PCR技术在肺外结核中的诊断价值

李秀武; 刘建强; 郑荣坤; 何萍; 杨志英; 李秋贞;   

  1. 河北省胸科医院;
  • 出版日期:2010-12-15 发布日期:2011-08-23
  • 基金资助:

    河北省2009年医学科学研究重点课题计划(20090260)

The diagnostic value of FQ-PCR in extra-pulmonary tuberculosis

Hebei chest hospital; Shijiazhuang 050041; China;   

  1. Li Xiuwu; Liu Jianqiang; Zheng Rongkun; He Ping; Yang Zhiying; Li Qiuzhen
  • Online:2010-12-15 Published:2011-08-23

摘要: 目的探讨FQ-PCR技术对活动性骨关节结核和泌尿系结核的诊断价值。方法选择118例疑似结核病患者,其中关节炎患者52例,泌尿系感染患者66例,应用FQ-PCR技术分别检测患者关节腔积液和尿液中的TB-DNA,并与抗酸染色法的检出率比较。结果 52例关节炎患者中,确诊结核性关节炎患者36例,TB-DNA阳性22例,22例全部为确诊病例,阳性率61.1%。抗酸染色的阳性数为2,均为TB-DNA阳性者,阳性率5.6%;66例泌尿系统疾病患者中,确诊泌尿系结核患者52例,TB-DNA阳性32例,32例全部为确诊病例,阳性率为61.5%,抗酸染色的阳性数为4,均为TB-DNA阳性者,阳性率7.7%。结论应用FQ-PCR技术检测患者关节腔积液和尿液中的TB-DNA,具有高度敏感性和特异性,可作为结核性关节炎和泌尿系统结核早期诊断的一个可靠指标。

关键词: 结核, 髋节/诊断, 泌尿生殖系统/诊断, 聚合酶链反应

Abstract: Objective To explore the value of FQ-PCR in the diagnosis of active osteoarticular tuberculosis and urinary tuberculosis.Methods One hundred and eighteen cases with suspected tuberculosis including 52 arthritis and 66 urinary tract infection were collected to detect TB-DNA using FQ-PCR and mycobacteria using acid-fast staining from joint effusion and urine versus detection rate of acid fast staining.Results Thirty-six of 52 cases with arthritis were diagnosed with tuberculosis.Among 36 cases,22 cases with p...

Key words: tuberculosis, osteoarticular/diagnosis, urogenital/diagnosis, polymerase chain reaction

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