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中国防痨杂志 ›› 2010, Vol. 32 ›› Issue (2): 109-113.

• 论著 • 上一篇    下一篇

北京市海淀区综合医疗机构抗酸染色镜检质量分析

叶国庆, 沈秀平, 钟春梅, 崔雅文, 陆巧英   

  1. 北京市海淀区疾病预防控制中心,
  • 出版日期:2010-02-20 发布日期:2010-02-20
  • 基金资助:

    基金资助:上海市公共卫生重点学科建设项目(08GWZX0103);上海市(复旦大学附属)公共卫生临床中心启动课题(200707)

Quality analysis of AFB microscopy in general hospitals in Haidian district of Beijing

Ye Guoqing,Shen Xiuping,Zhong Chunmei,Cui Yawen,Lu Qiaoying   

  1. Center for Disease Control and Prevention of Haidian District,Beijing 10094,China
  • Online:2010-02-20 Published:2010-02-20
  • Contact: Ye Guoqing E-mail:yeguoqing1001@126.com

摘要: 目的 评价北京市海淀区综合医疗机构痰涂片抗酸染色镜检质量,提出提高检查质量的措施。 方法 按照《中国结核病防治规划—痰涂片镜检质量保证手册》(EQA)的要求,对辖区开展抗酸染色检查的综合医疗机构检验科进行操作方法、结果判定和报告的标准化技术培训。同时,对相应机构开展的抗酸染色检查项目进行现场调查、评价和盲法复检。 结果综合医疗机构之间抗酸染色工作量差异显著。二级、三级综合医疗机构间抗酸染色检出率差异无统计学意义。复检定性符合率97.7%、阳性结果定量符合率93.6%。 结论 综合医疗机构AFB镜检能力质量存在差异,需进一步提高AFB镜检的能力和质量。

关键词: 染色与标记, 显微镜检查, 质量控制

Abstract: Objective To evaluate the quality of AFB microscopy in general hospitals in Haidian district of Beijing and propose measures to improve the quality. Methods The technicians from 11 general hospitals were trained for standard operational procedure and Results interpretation and reporting of AFB examination. The method of EQA was done according to the quality assurance manual of NTP. Meanwhile, on-site investigation, evaluation and blinded reexamination was carried out to general hospitals.  Results There is significance difference in terms of workloads between different hospitals. Positive rates of AFB were not significant different between class-2 and class-3 hospitals. For reexamination, qualitative consistency rate was 97.7%, and quantitative consistency rate was 93.6%. Conclusion The quality of AFB microscopy varies between different general hospitals, therefore the capacity and quality should be further improved.

Key words: staining and labeling, microscopy, quality control