耐多药结核分枝杆菌异烟肼耐药相关基因katG突变分析

中国防痨杂志 ›› 2013, Vol. 35 ›› Issue (3): 207-209.

耐多药结核分枝杆菌异烟肼耐药相关基因katG突变分析

甄伟石大伟赵玉玲李辉李亮朱国峰

100176 北京，中国医学科学院北京协和医学院病原生物学研究所卫生部病原系统生物学重点实验室（甄伟、朱国峰）；中国食品药品检定研究院体外诊断试剂二室（石大伟）；河南省疾病预防控制中心结核科（赵玉玲、李辉）；北京结核病胸部肿瘤研究所（李亮）

收稿日期:2012-12-22 出版日期:2013-03-10 发布日期:2013-03-14
通信作者: 朱国峰 E-mail:zhugf@yahoo.com
基金资助:
中澳卫生与艾滋病项目（EID23）；北京市科委对接项目(D08050700640801)

Received:2012-12-22 Online:2013-03-10 Published:2013-03-14

摘要/Abstract

摘要： 异烟肼(isoniazid, INH)是一种重要的抗结核药物，其在细菌体内被过氧化氢酶-过氧化物酶KatG氧化为乙酰异烟肼自由基和活性氧自由基等活性物质，进而攻击结核分枝杆菌多个靶位点来发挥杀菌作用。Zhang等通过Southern印迹杂交（Southern blot）发现结核分枝杆菌katG基因缺失和异烟肼耐药相关。研究表明，异烟肼耐药与许多基因相关，发生频率最高的为katG编码区和inhA启动子区的突变，katG315位突变最常见,有30%～94%的耐药株发生该突变，但国内对于该基因其他位点的突变研究较少。本研究通过对河南省耐多药(multidrug resistant,MDR)结核分枝杆菌katG编码区全长突变进行分析，进一步分析异烟肼的耐药机制。

甄伟石大伟赵玉玲李辉李亮朱国峰. 耐多药结核分枝杆菌异烟肼耐药相关基因katG突变分析[J]. 中国防痨杂志, 2013, 35(3): 207-209.

. [J]. Chinese Journal of Antituberculosis, 2013, 35(3): 207-209.

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