夹层杯离心涂片集菌法快速检测抗酸杆菌临床应用的初步观察

中国防痨杂志 ›› 2009, Vol. 31 ›› Issue (9): 535-537.

夹层杯离心涂片集菌法快速检测抗酸杆菌临床应用的初步观察

王淑侠；刘成永；闫家微；成松；张瑞梅；侯远沛

徐州市传染病医院徐州 221004

出版日期:2009-09-10 发布日期:2011-11-03

Primary observation of fast detection acid-fast bacilli by centrifuging the liquefied sputa and collecting bacteria

Wang Shuxia,Liu Chengyong,Yan Jiawei,Cheng Song,Zhang Ruimei,Hou Yuanpei

The infectious disease hospital,Xuzhou221004,China

Online:2009-09-10 Published:2011-11-03

摘要/Abstract

摘要： 目的研究夹层杯离心涂片集菌法快速检测抗酸杆菌的实用价值。方法夹层杯离心涂片集菌法和直接涂片法对结核门诊病人痰标本的阳性检出率进行比较；并对住院确诊肺结核病人的痰标本与罗氏培养法比较。结果结防门诊病人痰240份，直接涂片法阳性率25.8％，夹层杯涂片离心集菌法痰涂片阳性率40.8％，2者比较差异有统计学意义。以罗氏培养法为标准，夹层杯涂片离心集菌法的敏感性、特异性、阳性预期值、阴性预期值及准确性分别为： 90.9％、93.8％、97.6％、78.9％和91.7％。结论夹层杯离心涂片集菌法是一种更利于标准化、简便化的抗酸杆菌检查方法，检出率优于直接涂片法，并与罗氏培养法相比有较满意的符合率。大大有利于检验工作人员的生物安全防护，提高检验水平。

关键词: 结核, 肺/诊断, 分枝杆菌, 结核, 集落计数, 微生物

Abstract: ObjectiveTo study the clinical value of fast detection acid-fast bacilli by centrifuging the liquefied sputa and collecting bacteria. MethodsCompared the positive rate of outpatient sputum samples which detected by centrifuging the liquefied sputa and collecting bacteria with that by conventional smear method simultaneously, and evaluated this method in comparison with L-J medium used to detect the sputum samples of inpatients. ResultsThe positive rate of 240 outpatient sputum samples by centrifuging the liquefied sputa and collecting bacteria and by conventional smear method were 40.8% and 25.8%, respectively. There was significant difference. Using L-J medium as the control, the sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 90.9%, 93.8%, 97.6%, 78.9% and 91.7% respectively. ConclusionsThe centrifuging the liquefied sputa and collecting bacteria is a clinical testing method, which is easy to be standardized, simplified and accurated.

Key words: Tuberculosis,pulmonary/diagnosis, Mycobacterium tuberculosis, Colony count,microbial

王淑侠,刘成永,闫家微,成松,张瑞梅,侯远沛. 夹层杯离心涂片集菌法快速检测抗酸杆菌临床应用的初步观察[J]. 中国防痨杂志, 2009, 31(9): 535-537.

Wang Shuxia,Liu Chengyong,Yan Jiawei,Cheng Song,Zhang Ruimei,Hou Yuanpei. Primary observation of fast detection acid-fast bacilli by centrifuging the liquefied sputa and collecting bacteria[J]. Chinese Journal of Antituberculosis, 2009, 31(9): 535-537.