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中国防痨杂志 ›› 2002, Vol. 24 ›› Issue (6): 313-316.

• 论著 •    下一篇

吉林市结核分支杆菌IS6110 DNA指纹图谱特征分析

李书琳；韩中波；徐新顺；张永胜；李昕；   

  1. 吉林市结核病防治研究所 吉林 132011；
  • 出版日期:2002-06-10 发布日期:2002-11-03
  • 基金资助:
    吉林省卫生厅医学科研基金 (2000-143)

The analysis of characteristics of IS6110 fingerprint patterns of Mycobacterium tuberculosis strains in Jilin City

LI Shu lin,HAN Zhong bo,XU Xin shun,et al.   

  1. Jilin City Tuberculosis Institute,Jilin 132011
  • Online:2002-06-10 Published:2002-11-03

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摘要： 目的　分析吉林市结核分支杆菌IS6110 RFLP图谱特征。方法 对53株结核分支杆菌分离株进行IS6110基因分型，得到IS6110 RFLP图谱；按菌株指纹特征的同源性高低予以分组，并进行分析。结果 吉林市结核分支杆菌IS6110 RFLP图谱特征为：(1)IS6110拷贝数目平均为13个；(2)A、B两组同源性很高，具有“北京基因型”特征，C组多态性较强；(3)IS6110 RFLP图谱特征分布无地域差异；(4)耐药菌株与敏感菌株图谱特征无明显差异，但初治耐药菌株成簇率较高。结论 吉林市结核分支杆菌IS6110RFLP图谱特征以“北京基因型”为主；但还具一些独有的特征。

关键词: 分支杆菌,结核, DNA指纹, 吉林市

Abstract: Objective To analyze the characteristics of IS6110 restriction fragment length polymorphism ( RFLP ) patterns of mycobacterium tuberculosis strains isolated from Jilin City.Methods 53 of Mycobacterium tuberculosis strains were isolated from the 53 cases with pulmonary tuberculosis,IS6110 RFLP patterns were produced by DNA fingerprinting. Mycobacterium tuberculosis strains were divided into groups according to their homology of IS6110 fingerprinting,and were analyzed.Results The characteristics of IS6110 RFLP patterns of Mycobacterium tuberculosis strains isolated from Jilin City showed:(1)The average of the copies of IS6110 was 13.(2)The homology of the strains between group A and group B existed to a great extent.They had the characteristic of "Beijing genotype strains".Strains in group C had high polymorphism.(3)The characteristics of IS6110 patterns were not related to the geographic distribution of the patients.(4)The clustering rate of strains in initial cases of drug resistance was high;as for the characteristic of the patterns,there were no differences between the strains of drug resistance and those of non drug resistance.Conclusion The main type of the IS6110 RFLP patterns of Mycobacterium tuberculosis strains in Jilin City was "Binjing genotype strains",but there was a feature specially,that was the high polymorphism.

Key words: Mycobacterium,tuberculosis, DNA fingerprint, Jilin city

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