采用发光分析方法进行结核分枝杆菌快速药敏试验

中国防痨杂志 ›› 2000, Vol. 22 ›› Issue (2): 102-105.

采用发光分析方法进行结核分枝杆菌快速药敏试验

吕斌；徐顺清；周宜开；

武汉同济医科大学环境医学研究所 430030；

收稿日期:1999-10-13 出版日期:2000-02-10 发布日期:2000-02-10

Received:1999-10-13 Online:2000-02-10 Published:2000-02-10

摘要/Abstract

摘要： 结核病的发病率及死亡率在全球范围内均有上升的趋势?据WHO统计,结核病是第四位的主要死因,99%的结核病死亡和95%的新病例发生在发展中国家?世界人口中有19亿人感染结核杆菌,而且由于获得性免疫系统缺陷综合症—艾滋病的流行,在非洲?美洲许多国家及某些亚洲国家出现结核杆菌和艾滋病毒双重感染的病人,这是结核病第三次回升的新动向?WHO预测,若不采取有效措施,未来的十年中,全球将有3000多万人死于结核病[1]?中国人口占世界人口的1/4,结核病患者的人数亦占世界结核病人数的1/4?

吕斌, 徐顺清, 周宜开, . 采用发光分析方法进行结核分枝杆菌快速药敏试验[J]. 中国防痨杂志, 2000, 22(2): 102-105.

参考文献

1 Shimao T. Global situation of TB and its control. Kekkaku,1999,74(2) :83-90.
2 严碧涯.警钟长鸣：防治结核病和爱滋病.中华结核和呼吸杂志，1996，19:131- 132.
3 Centers for Disease Control and Prevention. Tuberculosis mor-bidity -United States. 1995. Morbid Mortal. Weekly Report,1996,45:365-370.
4 Teng LJ,Chung PF,Liaw SJ,et al.A rapid method for deter-mination of susceptibility of Mycobacterium tuberculosis to iso-niazid,using acridinium-ester-labeled DNA probe. Chung Hua Min Kuo Wei Sheng Wu Chi Mien I Hsueh Tsa Chih，1994,27(3)：103-112.
5 Tomioka H, Sato K，Saito H. In vitro susceptibilities of Myco,bacterium avium and Mycobacterium intracellulare to various drugs. Kekkaku ,1991,66:489-492.
6 Moore DF,Curry JI,Knott CA ,iet al. Amplification of rRNA for assessment of treatment response of pulmonary tuberculosis pa-tients during antimicrobial therapy, J Clin Microbiol, 1996,34:1745-1749.
7 McNerney R. TB ： the return of the phage. A review of fifty years of mycobacteriphage research.Int J Tuberc Lung Dia,1999,3:179-184.
8 Jacobs WR, Barletla RG,Udani R,et al. Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages.Science, 1993，260:819-822.
9 Carriere C, Riska P,Zimhony O，el al. Conditional replicating luciferase reporter phages: improved sensitivity for rapid detec-tion and assessment of drug susceptibility of Mycobacterium tuberculosis.J.Clinic Microb, 1997,35:3232-3239.
10 Riska PF, Jacobs WR, Bloom BR, et al.Specific identification of Mycobacterium tuberculosis with the luciferase reporter my-cobacteriophage：use of p-nitro-acetylaraino-β-hydrocxy propiophenone, J.Clinic Microbio, 1997,35:3225-3231.
11 Riska PF,Su Y, Bardarov S,et al. Rapid film-based determi-nation of antibiotic susceptibilities of Mycobacterium tubercu-losis strains by using a luciferase reporter phage and bronx box.J Clin Microbiol. 1999,37： 1144-1149.
12 Cooksey RC,Crawford JT,Jacobs WR，et al. A rapid method for screening antimicrobial agents for activities against a strain of Mycobacerium tuberculosis expressing firefly luciferase.An-timicrob Agents Chemother, 1993,37： 1348-1352.
13 Fullner KJ，Halfull GF. Mycobacteriophage L5 infection (A my-cobacterium bovis BCG: implication for phage genetics in the slow-growing mycobacteria. Mol Microbiol, 1997,26:755-766.
14 Nilsson LE, HofTner SE, Ansehn S. Rapid susceptibility testing of Mycobacterium tuberculosis by bioluminescence assay of mycobacterial ATP. Antimicrob Agents Chemother, 1988,32:1208-1212.
15 Limb DI, Wheat PF, Spencer RC, et al.Comparison of tech-niques for antimicrobial susceptibility testing of mycobacteria.J Clin Pathol,1993,46:403-407.
16 Yamazaki T, Sato N, Yamashita K, et al.Antimicrobial suscep-tibility testing for Mycobacterium tuberculosis by measuring mycobacterial adenosine triphosphate. Rinaho Byori, 1999,47:170-175.
17 Beckera B,Lang HR,Schimke D,et al.Evaluation d a biolu-minescence assay fw rapid antimicrobial susceptibility testing of mycobacteria. Eur J Clin Microbiol, 1985,4：556-561.