Research progress of relationship between CRISPR-Cas system and Mycobacterium tuberculosis

doi:10.3969/j.issn.1000-6621.2021.12.018

Abstract

Abstract:

Clustered regularly interspaced short palindromic repeats (CRISPR) and their related proteins (Cas) form a new acquired immune defense system distributed in Mycobacterium tuberculosis (MTB). In addition, CRISPR-Cas system may have an important relationship with the virulence, drug resistance and targeted gene editing of MTB. This paper reviews the immune function of CRISPR-Cas system in MTB and its related research progress, in order to provide a new idea for further study of MTB pathogenicity and anti-tuberculosis treatment.

Key words: Mycobacterium tuberculosis, CRISPR-Cas system, Drug resistance, Transcription

YANG Rui-fang, LI Chuan-you. Research progress of relationship between CRISPR-Cas system and Mycobacterium tuberculosis[J]. Chinese Journal of Antituberculosis, 2021, 43(12): 1332-1335. doi: 10.3969/j.issn.1000-6621.2021.12.018

References 36

[1]	Khawbung JL, Nath D, Chakraborty S. Drug resistant Tuberculosis: A review. Comp Immunol Microbiol Infect Dis, 2021, 74:101574. doi: 10.1016/j.cimid.2020.101574. doi: 10.1016/j.cimid.2020.101574 URL
[2]	Hryhorowicz M, Lipinski D, Zeyland J, et al. CRISPR/Cas9 Immune System as a Tool for Genome Engineering. Arch Immunol Ther Exp (Warsz), 2017, 65(3):233-240. doi: 10.1007/s00005-016-0427-5. doi: 10.1007/s00005-016-0427-5 URL
[3]	Liu L, Fan XD. CRISPR-Cas system: a powerful tool for genome engineering. Plant Mol Biol, 2014, 85(3):209-218. doi: 10.1007/s11103-014-0188-7. doi: 10.1007/s11103-014-0188-7 URL
[4]	Koonin EV, Makarova KS. Origins and evolution of CRISPR-Cas systems. Philos Trans R Soc Lond B Biol Sci, 2019, 374(1772):20180087. doi: 10.1098/rstb.2018.0087. doi: 10.1098/rstb.2018.0087 URL
[5]	Koonin EV, Makarova KS. Mobile Genetic Elements and Evolution of CRISPR-Cas Systems:All the Way There and Back. Genome Biol Evol, 2017, 9(10):2812-2825. doi: 10.1093/gbe/evx192. doi: 10.1093/gbe/evx192 URL
[6]	翟小倩, 鲍朗. CRISPR-Cas系统及其在结核分枝杆菌中的研究进展. 生物学杂志, 2018, 35(1):89-92. doi: 10.3969/j.issn.2095-1736.2018.01.089. doi: 10.3969/j.issn.2095-1736.2018.01.089
[7]	Wei J, Lu N, Li Z, et al. The Mycobacterium tuberculosis CRISPR-Associated Cas1 Involves Persistence and Tolerance to Anti-Tubercular Drugs. Biomed Res Int, 2019, 2019:7861695. doi: 10.1155/2019/7861695. doi: 10.1155/2019/7861695
[8]	冯欢欢, 单彩龙, 李金月, 等. CRISPR系统中Cas蛋白的分类及作用机制. 中国病原生物学杂志, 2018, 13(6):652-654, 663. doi: 10.13350/j.cjpb.180624. doi: 10.13350/j.cjpb.180624
[9]	Chen H, Zhang SD, Chen L, et al. Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior. Front Microbiol, 2018, 9:1569. doi: 10.3389/fmicb.2018.01569. doi: 10.3389/fmicb.2018.01569 URL
[10]	黄琴琴. 结核分枝杆菌Cas2(Rv2816c)在胁迫应答及胞内存活中的作用与分子机理. 重庆:西南大学, 2015.
[11]	Killelea T, Bolt EL. CRISPR-Cas adaptive immunity and the three Rs. Biosci Rep, 2017, 37(4):BSR20160297. doi: 10.1042/BSR20160297. doi: 10.1042/BSR20160297
[12]	Plagens A, Richter H, Charpentier E, et al. DNA and RNA interference mechanisms by CRISPR-Cas surveillance complexes. FEMS Microbiol Rev, 2015, 39(3):442-463. doi: 10.1093/femsre/fuv019. doi: 10.1093/femsre/fuv019 pmid: 25934119
[13]	Wei W, Zhang S, Fleming J, et al. Mycobacterium tuberculosis type Ⅲ-A CRISPR/Cas system crRNA and its maturation have atypical features. FASEB J, 2019, 33(1):1496-1509. doi: 10.1096/fj.201800557RR. doi: 10.1096/fj.201800557RR URL
[14]	Larson MH, Gilbert LA, Wang X, et al. CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nat Protoc, 2013, 8(11):2180-2196. doi: 10.1038/nprot.2013.132. doi: 10.1038/nprot.2013.132 URL
[15]	Gilbert LA, Larson MH, Morsut L, et al. CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell, 2013, 154(2):442-451. doi: 10.1016/j.cell.2013.06.044. doi: 10.1016/j.cell.2013.06.044 pmid: 23849981
[16]	Qi LS, Larson MH, Gilbert LA, et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell, 2013, 152(5):1173-1183. doi: 10.1016/j.cell.2013.02.022. doi: 10.1016/j.cell.2013.02.022 URL
[17]	刘雅婷. 结核分枝杆菌Cas蛋白表达纯化与功能研究. 郑州:郑州大学, 2012.
[18]	Zhang Y, Yang J, Bai G. Regulation of the CRISPR-Associated Genes by Rv2837c (CnpB) via an Orn-Like Activity in Tuberculosis Complex Mycobacteria. J Bacteriol, 2018, 200(8):e00743-17. doi: 10.1128/JB.00743-17. doi: 10.1128/JB.00743-17
[19]	Liu Z, Dong H, Cui Y, et al. Application of different types of CRISPR/Cas-based systems in bacteria. Microb Cell Fact, 2020, 19(1):172. doi: 10.1186/s12934-020-01431-z. doi: 10.1186/s12934-020-01431-z URL
[20]	Sander JD, Joung JK. CRISPR-Cas systems for editing, regulating and targeting genomes. Nat Biotechnol, 2014, 32(4):347-355. doi: 10.1038/nbt.2842. doi: 10.1038/nbt.2842 URL
[21]	Cong L, Ran FA, Cox D, et al. Multiplex genome engineering using CRISPR/Cas systems. Science, 2013, 339(6121):819-823. doi: 10.1126/science.1231143. doi: 10.1126/science.1231143 pmid: 23287718
[22]	Li Y, Peng N. Endogenous CRISPR-Cas System-Based Genome Editing and Antimicrobials:Review and Prospects. Front Microbiol, 2019, 10:2471. doi: 10.3389/fmicb.2019.02471. doi: 10.3389/fmicb.2019.02471 URL
[23]	Yan MY, Li SS, Ding XY, et al. A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis. mBio, 2020, 11(1):e02364-19. doi 10.1128/mBio.02364-19. doi: 10.1128/mBio.02364-19
[24]	Rock J. Tuberculosis drug discovery in the CRISPR era. PLoS Pathog, 2019, 15(9):e1007975. doi: 10.1371/journal.ppat.1007975. doi: 10.1371/journal.ppat.1007975 URL
[25]	Rock JM, Hopkins FF, Chavez A, et al. Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform. Nat Microbiol, 2017, 2:16274. doi: 10.1038/nmicrobiol.2016.274. doi: 10.1038/nmicrobiol.2016.274 URL
[26]	郭越, 赵增祥, 张文姝, 等. 基因编辑调控技术在结核分枝杆菌基因功能研究中的应用进展. 中国病原生物学杂志, 2020, 15(4):483-486. doi: 10.13350/j.cjpb.200425. doi: 10.13350/j.cjpb.200425
[27]	Singh A, Gaur M, Sharma V, et al. Comparative Genomic Analysis of Mycobacteriaceae Reveals Horizontal Gene Transfer-Mediated Evolution of the CRISPR-Cas System in the Mycobacterium tuberculosis Complex. mSystems, 2021, 6(1):e00934-20. doi: 10.1128/mSystems.00934-20. doi: 10.1128/mSystems.00934-20
[28]	Zhang S, Li T, Huo Y, et al. Mycobacterium tuberculosis CRISPR/Cas system Csm1 holds clues to the evolutionary relationship between DNA polymerase and cyclase activity. Int J Biol Macromol, 2021, 170:140-149. doi: 10.1016/j.ijbiomac.2020.12.014. doi: 10.1016/j.ijbiomac.2020.12.014 URL
[29]	Gunderson FF, Cianciotto NP. The CRISPR-associated gene cas2 of Legionella pneumophila is required for intracellular infection of amoebae. mBio, 2013, 4(2):e00074-13. doi: 10.1128/mBio.00074-13. doi: 10.1128/mBio.00074-13
[30]	Lam JT, Yuen KY, Ho PL, et al. Truncated Rv2820c enhances mycobacterial virulence ex vivo and in vivo. Microb Pathog, 2011, 50(6):331-335. doi: 10.1016/j.micpath.2011.02.008. doi: 10.1016/j.micpath.2011.02.008 URL
[31]	Rindi L, Lari N, Garzelli C. Genes of Mycobacterium tuberculosis H37Rv down regulated in the attenuated strain H37Ra are restricted to M.tuberculosis complex species. New Microbiol, 2001, 24(3):289-294. pmid: 11497087
[32]	吴晓丽. 结核分枝杆菌Csm3蛋白功能的初步研究. 福州:福建农林大学, 2016.
[33]	Makarova KS, Wolf YI, Alkhnbashi OS, et al. An updated evolutionary classification of CRISPR-Cas systems. Nat Rev Microbiol, 2015, 13(11):722-736. doi: 10.1038/nrmicro3569. doi: 10.1038/nrmicro3569 pmid: 26411297
[34]	Hatfull GF. Molecular Genetics of Mycobacteriophages. Microbiol Spectr, 2014, 2(2):1-36. doi: 10.1128/microbiolspec.MGM2-0032-2013. doi: 10.1128/microbiolspec.MGM2-0032-2013 pmid: 25328854
[35]	Ren L, Deng LH, Zhang RP, et al. Relationship between drug resistance and the clustered, regularly interspaced, short, palindromic repeat-associated protein genes cas1 and cas2 in Shigella from giant panda dung. Medicine (Baltimore), 2017, 96(7):e5922. doi: 10.1097/MD.0000000000005922. doi: 10.1097/MD.0000000000005922 URL
[36]	Brudey K, Driscoll JR, Rigouts L, et al. Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology. BMC Microbiol, 2006, 6:23. doi: 10.1186/1471-2180-6-23. doi: 10.1186/1471-2180-6-23 URL