4-nitrobenzoic acid experiment of mycobacteria and research progress on its molecular mechanism of Mycobacterium differentiation

doi:10.19982/j.issn.1000-6621.20230191

Abstract

Abstract:

Mycobacterial disease is an important infectious disease threatening human health worldwide. The diseases caused by Mycobacterium tuberculosis (MTB) and non-tuberculous mycobacteria (NTM) are very similar in clinical symptoms, pathological manifestations and imaging features. However, there are inherent differences in the drug resistance spectrum between these two types of strains, resulting in significant differences in clinical treatment and management, therefore, rapid and accurate differentiation method is needed. PNB (4-nitrobenzoic acid) experiment is a selective culture method based on the different susceptibility of mycobacteria to PNB. It is widely used in clinical practice due to its simplicity, low price, and low technical requirements, but the mechanism involved in this experiment is unclear. This article reviewed the PNB experimental methods, reported researches on the PNB metabolic enzymes of mycobacteria and existing problems to provide ideas for elucidating the mechanism of mycobacteria PNB resistance, the principle of distinguishing MTB from NTM, and the new application value of PNB.

Key words: Nitrobenzenes, Mycobacterium tuberculosis, Non-tuberculous mycobacterium

CLC Number:

R378.91

Li Yue, Xia Hui, Li Machao, Wang Ruibai. 4-nitrobenzoic acid experiment of mycobacteria and research progress on its molecular mechanism of Mycobacterium differentiation[J]. Chinese Journal of Antituberculosis, 2023, 45(10): 997-1002. doi: 10.19982/j.issn.1000-6621.20230191

Figures/Tables 2

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方法名称	基质类型	培养基	PNB浓度	样本量	报告时间
传统法	固体	罗氏培养基	500μg/ml	100μl 10^-1mg/ml	28d
PNB-MGIT	液体	BACTEC MGIT 960	500μg/ml	1麦氏浊度, 1∶5稀释后接种500μl	7~10d
微量肉汤法		Middlebrook 7H9 ( 10%OADC)	200μg/ml	10^-3mg/ml,100μl接种	7~10d
PNB-7H10	固体	Middlebrook 7H10 (10%OADC)	500μg/ml	1麦氏浊度,1∶100稀释后10μl接种	3~28d
NRAp		罗氏培养基(1000μg/ml KNO₃)	500μg/ml	200μl痰液接种,1ml Griess显色	7~10d
分光光度计	代谢产物	水	0.001%	20mg/ml	8h
LC-MS		水	0.001%	20mg/ml	8h