肺结核患者血清视黄醇和视黄醇结合蛋白4水平变化的研究

doi:10.3969/j.issn.1000-6621.2015.02.003

中国防痨杂志 ›› 2015, Vol. 37 ›› Issue (2): 128-133.doi: 10.3969/j.issn.1000-6621.2015.02.003

肺结核患者血清视黄醇和视黄醇结合蛋白4水平变化的研究

麦洪珍杨智宋晓东蔡春葵路希维

116033 大连市结核病医院结核内科

收稿日期:2014-10-23 出版日期:2015-02-10 发布日期:2015-03-21
通信作者: 宋晓东 E-mail:xiaodongdong63@gmail.com
基金资助:
大连市卫生局科研基金项目(20110729)

Study on levels of serum retinol and retinol-binding protein-4 in patients with pulmonary tuberculosis

MAI Hong-zhen, YANG Zhi, SONG Xiao-dong, CAI Chun-kui, LU Xi-wei

Department of Medicine, Dalian Tuberculosis Hospital, Dalian 116033, China

Received:2014-10-23 Online:2015-02-10 Published:2015-03-21
Contact: SONG Xiao-dong E-mail:xiaodongdong63@gmail.com

摘要/Abstract

摘要： 目的检测耐药肺结核、非耐药肺结核患者和健康对照者血清维生素A（视黄醇）和视黄醇结合蛋白4（retinol-binding protein-4，RBP₄）水平，并分析它们的影响因素。方法收集2011年7月至2013年8月大连市结核病医院门诊随访及住院治疗的耐药肺结核患者128例（耐药组）、非耐药肺结核患者152例 (非耐药组)，匹配选取我院同期健康体检者120名作为对照组。采用高效液相色谱法（HPLC）测定上述受试者血清视黄醇水平，同时采用酶联免疫吸附测定法（ELISA）检测血清RBP₄水平。结果以（x±s）表示，所有数据采用SPSS 15.0统计软件进行统计学分析。多组间比较采用单因素方差分析及SNK-q检验，以P<0.05为差异有统计学意义。影响因素分析采用直线相关和多元逐步回归分析。结果耐药组、非耐药组患者血清视黄醇、RBP₄水平分别为（206.10±10.35）μg/L、（6.22±1.64）μg/ml和（249.61±12.06）μg/L、（8.23±2.31）μg/ml，均显著低于对照组血清视黄醇、RBP₄水平（326.57±11.52）μg/L、（11.52±2.60）μg/ml，差异均有统计学意义（q=12.35和 10.66,P值均＜0.01；q=3.86和3.36, P值均＜0.05）；而耐药组较非耐药组，患者血清视黄醇、RBP₄水平均显著降低，差异均有统计学意义（q=3.25和3.12, P值均＜0.05）。多元逐步回归分析表明，体质量指数（BMI）是血清视黄醇和RBP₄的独立影响因素（t=2.154和5.211，P值均＜0.05）。结论肺结核患者血清视黄醇和RBP₄水平均显著降低,耐药肺结核患者降低更明显。

关键词: 结核, 肺/血液, 维生素A, 视黄醇结合蛋白质类

Abstract: Objective To determine the levels of serum vitamin A (retinol) and retinol-binding protein-4 (RBP₄) in drug-resistant pulmonary tuberculosis (PTB), susceptible PTB and healthy people; to analyze the related factors to influence the levels of serum retinol and RBP₄. Methods Outpatients and inpatients with PTB who received treatment in Dalian TB Hospital from July 2011 to August 2013 were recruited in this study, including 128 drug-resistant PTB patients (drug-resistant group) and 152 susceptible PTB patients (non drug-resistant group). Meanwhile, 120 matched healthy persons who received physical examinations in the same hospital were selected as the control group. The levels of serum retinol and serum RBP₄ among all those recruited subjects were tested. The serum retinol was measured by high-performance liquid chromatography (HPLC) and the serum RBP₄ was measured by enzyme-linked immunosorbent assay (ELISA). The results were showed as x±s and all data were analyzed using SPSS software version 15.0. ANOVA and SNK-q test were used for the comparisons among multi groups. P<0.05 was regarded as statistical significance. Linear correlation and multiple stepwise regression analysis were used for correlated factors analysis of the levels serum retinol and RBP₄. Results The levels of serum retinol and RBP₄ in drug-resistant group and non drug-resistant group were (206.10±10.35) μg/L, (6.22±1.64) μg/ml and (249.61±12.06) μg/L, (8.23±2.31) μg/ml respectively, which were significantly lower than those in the control group ((326.57±11.52) μg/L and (11.52±2.60) μg/ml) (q=12.35, q=10.66, all P<0.01；q=3.86, q=3.36, all P<0.05); Furthermore, the levels of serum retinol and RBP₄ in drug-resistant group were significantly lower than those in non drug-resistant group (q=3.25, q=3.12, all P<0.05). A multiple stepwise regression analysis showed that body mass index (BMI) was the only independent influencing factor of the levels of serum retinol and RBP₄ (t=2.154, t=5.211, all P<0.05). Conclusion The levels of serum retinol and RBP₄ significantly decreased in PTB patients and the decrease was more obvious in drug-resistant PTB patients.

Key words: Tuberculosis, pulmonary/Blood, Vitamin A, Retinol-binding proteins

麦洪珍，杨智，宋晓东，蔡春葵，路希维. 肺结核患者血清视黄醇和视黄醇结合蛋白4水平变化的研究[J]. 中国防痨杂志, 2015, 37(2): 128-133. doi: 10.3969/j.issn.1000-6621.2015.02.003

MAI Hong-zhen, YANG Zhi, SONG Xiao-dong, CAI Chun-kui, LU Xi-wei. Study on levels of serum retinol and retinol-binding protein-4 in patients with pulmonary tuberculosis[J]. Chinese Journal of Antituberculosis, 2015, 37(2): 128-133. doi: 10.3969/j.issn.1000-6621.2015.02.003

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肺结核患者血清视黄醇和视黄醇结合蛋白4水平变化的研究

Study on levels of serum retinol and retinol-binding protein-4 in patients with pulmonary tuberculosis

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