关键词: 分枝杆菌, 结核, 微生物敏感性试验, 利福平, 异烟肼, 表型, 基因型, 寡核苷酸序列分析

Abstract: Objective Analysis drug susceptibility phenotype and genotype of Mycobacterium tuberculosis (Mtb) to rifampin(RFP) and isoniozid(INH). Methods One hundred and thirty-seven morning sputum specimens were cultured for Mycobacterium tuberculosis at Shenzhen Third People’s Hospital from 2009 to 2012, Use membrane gene chip test technology to detect the mutation type of RFP and INH resistance gene, absolute concentration drug susceptibility test were performed at the same time, calculate the coincidence rate between membrane gene chip test results and the absolute concentration drug susceptibility test, to verify the accuracy of the membrane gene chip method. Results The ratio of phenotype drug-resistant for RFP and INH were 27.01(37/137) and 22.63%(31/137), the ratio of genotype drug-resistant for RFP and INH were 26.28(36/137) and 23.36%(32/137). Compared with the gold standard of absolute concentration drug susceptibility test, the membrane gene chip method for RFP displayed the sensitivity 83.78%(31/37), specificity 94.06%（95/101）,concordance 91.97% (126/137),the value of Kappa was 0.79, P<0.05;INH displayed the sensitivity 80.65%(25/31), specificity 94.29%（99/105）, concordance 90.51% (124/137),the value of Kappa was 0.73, P<0.05; the total concordances were 91.24% (250/274) for the two drugs. Conclusion The membrane gene chip susceptibility results and absolute concentration method susceptibility results are highly consistent, which is adapted for extensive application in clinical treatment, as it allows fast and accuracy detection of the resistance to RFP and INH in Mtb clinical isolates.

Key words: Mycobacterium tuberculosis, Microbial sensitivity tests, Rifampin, Isoniazid, Phenotype, Genotype, Oligonucleotide array sequence analysis