关键词: 分枝杆菌, 结核, 培养基, 微生物敏感性试验, 卷曲霉素硫酸盐, 色谱法, 高压液相

Abstract: Objective To detect the stability of capreomycin (Cm) in the Roche medium, and find out the degradation rate of Cm in Roche medium and to detect the influence of Cm degradation on drug sensitive test. Methods The high performance liquid chromatograph（HPLC） test was used to detect the stability of Cm under preservation condition of 4 ℃ and 37 ℃ in Roche medium; Cm under different preservation condition was used to conduct drug sensitive test (DST) to detect the influence of Cm degradation on drug sensitive test. Results Preserved in 4 ℃, the concentration of Cm in Roche medium is inversely proportional to time, the degradation of Cm in Roche medium is close to 2.04% after 10 days, the degradation is 21.5% after 40 days, the degradation is 41.7% after 80 days, and Cm was not detected after 200 days. Preserved in 37 ℃, the degradation of Cm in Roche medium is close to 46.8% after 10 days, and Cm was not detected after 30 days. In the Cm Roche medium preserved at 4 ℃ for 10-160 days, DST results showed that in the strain which minimal inhibitory concentration (MIC) is between 0.1 to 0.2 μg/ml, 16 colonies were observed after 6 weeks in the Roche medium preserved in 4 ℃ for 40 days. In the strain which MIC is between 1.0 to 1.5 μg/ml, positive result (+) was reported after 6 weeks in the Roche medium preserved in 4 ℃ for 40 days. Conclusion Cm Roche medium is unstable when stored at 37 ℃, it should be preserved at 4 ℃ and should be used within 40 days.

Key words: Mycobacterium tuberculosis, Culture media, Microbial sensitivity tests, Capreomycin sulfate, Chromatography, high pressure liquid