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中国防痨杂志 ›› 2000, Vol. 22 ›› Issue (1): 26-27.

• 论著 • 上一篇    下一篇

胸膜活检标本行基因扩增对结核性胸膜炎的诊断价值

张玲莉;刘云梅;纪宪;翟守恒;韩通运;周素芬;杜志平;   

  1. 解放军264医院 太原030001;
  • 收稿日期:1999-08-11 修回日期:1999-10-26 出版日期:2000-01-10 发布日期:2000-01-10

Clinical value of PCR to pleural biopsy specimen in diagnosis of tuberculous pleuritis

ZHANG Lingli,LIU Yunmei,JI Xian,et al.   

  1. The 264th Hospital,PLA,Taiyuan 030001
  • Received:1999-08-11 Revised:1999-10-26 Online:2000-01-10 Published:2000-01-10

摘要: 目的 评价胸膜活检组织行聚合酶链反应(PCR)对结核性胸膜炎的诊断价值?方法 PCR检测65例胸膜活检组织中结核分枝杆菌DNA,并与胸水检测及胸膜活检组织病检对比?结果 胸膜活检组织PCR阳性率831%,胸水PCR阳性率为631%,胸膜活检组织病检阳性率为60.0%?前者较后两者更敏感?结论 胸膜活检组织PCR检测对结核性胸膜炎有较高的诊断价值?

关键词: 结核,胸膜, 聚合酶链反应, 组织学技术

Abstract: Objective To evaluate the value of PCR to pleural biopsy specimen in the diagnosis of tuberculous pleuritis.Methods PCR technique was used to detect M.tuberculosis DNA in pleural biopsy specimen of 65 cases.The results were compared with that of pleural liquid PCR and pleural pathological examination respectively.Results The positive rate was 83.1%by pleural biopsy specimen PCR,63.1% by pleural liquid PCR and 60.0%by pleural pathological examination respectively.This method was more sensitive than the two others.Conclusion PCR detection of pleural biopsy specimen had greater diagnostic value in tuberculous pleuritis.

Key words: Tuberculosis, pleural, Polymerase chain reaction, Histological technique