几种结核分支杆菌重组蛋白诱发豚鼠迟发型超敏反应的研究

中国防痨杂志 ›› 2002, Vol. 24 ›› Issue (3): 116-118.

几种结核分支杆菌重组蛋白诱发豚鼠迟发型超敏反应的研究

张灵霞；吴雪琼；史迎昌；梁建琴；张俊仙；

解放军309医院结核病研究室北京 100091；

出版日期:2002-03-10 发布日期:2002-11-03

Study on DTH reaction of guinea-pig induced by several recombinant antigen of M.tuberculosis

ZHANG Ling-xia,WU Xue-qiong,SHI Ying-chang,et al.

Tuberculosis Research Lab.309 Hospital of PLA,Beijing 100091

Online:2002-03-10 Published:2002-11-03

摘要/Abstract

摘要： 目的　了解结核分支杆菌重组抗原ESAT6 (E)、MPT64 (M)、Ag85A(A)及两两混合物诱发豚鼠产生迟发型超敏 (DTH)反应的能力。方法常规皮肤实验法 :灭活H₃₇R_v全菌体致敏豚鼠，皮肤试验以生理盐水为阴性对照，5UPPD标准品为阳性对照，0.8μgE、0.8μgM、0.8μgA、0.4μgA+0.4μgM、0.4μgA+0.4μgE、0.4μgE+0.4μgM分别于背部皮内注射，注射后24、48、72h分别测量硬结反应横、纵直径 (mm)，取二者的平均值。皮肤试验轮圈法 :生理盐水、5UPPD及0.8μgE、0.8μgM、0.8μgA、0.4μgA+0.4μgM、0.4μgA+0.4μgE、0.4μgE+0.4μgM，以轮圈法注射于同一只豚鼠背部两侧，每批6只，共3批。注射后24、48、72h分别测量硬结反应横、纵直径 (mm)，取二者的平均值。结果常规皮肤试验0.8μgM、A、M+E及E+A诱发豚鼠DTH的平均直径与PPD相比无显著性差异 (P＞0.05)，而0.8μgE抗原及M+A诱发豚鼠DTH的平均直径与PPD相比有显著性差异(P＜0.05)。时间效应 :重组抗原及其混合物诱发豚鼠DTH的平均直径24h平均直径显著高于48h平均直径 (P＜0.05)，24h测量效果较好。轮圈法皮肤试验排除个体差异后，结果与常规皮肤试验结果一致。结论从试验结果看，重组ESAT6抗原及MTP64与Ag85A混合物诱发豚鼠DTH反应强于PPD。

关键词: 分支杆菌,结核, 重组结核分支杆菌蛋白抗原, 迟发超敏反应

Abstract: Objective To observe the DTH reaction induced by recombinant antigen of M.tuberculosis MPT64(M)、Ag85A(A)、ESAT6(E) and the mixture of two of them.Methods Normal skin test:The guinea-pig were sensitived by all body of dead H₃₇R_v.We used water as negative control,5U PPD as positive control,0.8μgE、0.8μgM、0.8μgA、0.4μgA+0.4μgM、0.4μgA+0.4μgE、0.4μgE+0.4μgM were injected on guinea-pig’s back respectively.The Results were observed 24、48、72h after injection.Alternative skin test:water、5U PPD、0.8μgE、0.8μgM、0.8μgA、0.4μgA+ 0.4μgM、0.4μgA+ 0.4μgE、0.4μgE+ 0.4μgM were injectecd on the back of the same guinea-pig.The Results were observed 24、48、72h respectively after injection.Results Normal skin test showed:the DTH reaction of guinea induced by 0.8μgM、A、M+E、E+A mixture respectively was no real difference with the DTH reaction induced by PPD (P＞0.05).But the DTH reaction induced by 0.8μgE and M+A mixture respectively was stronger than the Results induced by PPD (P＜0.05).24h after injection was the best observing time of the test.The Results of alternative skin test were coincidence with normal skin test.Conclusion The DTH reaction induced by ESAT6 and A+M compounds was stronger than the DTH Results induced by PPD.

Key words: M.tuberculosis, Recombinant antigen, DTH

张灵霞,吴雪琼,史迎昌,梁建琴,张俊仙,. 几种结核分支杆菌重组蛋白诱发豚鼠迟发型超敏反应的研究[J]. 中国防痨杂志, 2002, 24(3): 116-118.

ZHANG Ling-xia,WU Xue-qiong,SHI Ying-chang,et al.. Study on DTH reaction of guinea-pig induced by several recombinant antigen of M.tuberculosis[J]. Chinese Journal of Antituberculosis, 2002, 24(3): 116-118.