结核性胸膜炎患者外周血CD4 CD25 调节性T细胞水平及Foxp3 mRNA表达的分析

中国防痨杂志 ›› 2009, Vol. 31 ›› Issue (10): 569-572.

结核性胸膜炎患者外周血CD4 CD25 调节性T细胞水平及Foxp3 mRNA表达的分析

吕伟¹；薛克营²；王成国²；张宏伟³

1.武汉市结核病防治所武汉 430030；2.郧阳医学院附属东风医院呼吸科十堰 442008；3.孝感市孝昌县第一人民医院孝感 432900

出版日期:2009-10-10 发布日期:2011-11-03

The analysis of CD4 CD25 regulatory T cells and Foxp3 mRNA in the peripheral blood from the patients with tuberculous pleurisy

Lv Wei¹, Xue Keying², Wang Chengguo², Zhang Hongwei³

1.Wuhan Institute of Tuberculosis Prevention and Control of Hubei Province, Wuhan 430030, China;2.Department of Respiration, the Affiliated Dongfeng Hospital, Yunyang Medical College, Shiyan 442008, China;3.The 1st People’s Hospital of Xiaochang, Xiaogan,432900,China

Online:2009-10-10 Published:2011-11-03

摘要/Abstract

摘要： 目的观察结核性胸膜炎患者外周血单个核细胞（PBMCs）中CD4 CD25调节性T细胞(CD4 CD25 Treg)水平及Foxp3 mRNA表达的变化，探讨CD4 CD25 Treg在结核性胸膜炎发病中的作用。方法采用流式细胞仪检测58例结核性胸膜炎患者和51例健康志愿者（正常对照组）PBMCs中CD4 CD25 Treg的比例；RT-PCR检测PBMCs中Foxp3 mRNA的表达。结果结核性胸膜炎患者PBMCs中CD4 CD25 Treg的比例明显高于正常对照组(P<0.05)；Foxp3 mRNA的表达明显高于正常对照组(P<0.05)。结论结核性胸膜炎患者外周血中具有免疫抑制活性CD4 CD25 Treg的数量增加，功能增强，可能是结核性胸膜炎发生发展的一个因素。

关键词: 结核，胸膜/血液, 白细胞，单核, T淋巴细胞，调节性, 叉头转录因子类

Abstract: ObjectiveTo investigate the changes of CD4 CD25 regulatory T cells (CD4 CD25 Treg) and Foxp3 mRNA in peripheral blood mononuclear cells (PBMCs) from the patients with tuberculous pleurisy, to elucidate the possible roles of CD4 CD25 Treg in the development of tuberculous pleurisy. MethodsThe peripheral blood samples were collected from 30 healthy controls (normal control group) and 32 patients with tuberculous pleurisy. The CD4 CD25 Treg ratio and Foxp3 mRNA in PBMCs were detected By flow cytometry and RT-PCR. ResultsThe CD4 CD25 Treg ratio and Foxp3 mRNA in PBMCs from tuberculous pleurisy group were higher than that from normal control group (P<0.05). ConclusionThe increase of CD4 CD25 Treg ratio and its function in PBMCs may be responsible for pathogenesis of tuberculous pleurisy.

Key words: tuberculosis,pleural/blood, leukocytes, mononuclear, T-lymphocytes,regulatory, forkhead transcription factors

吕伟,薛克营,王成国,张宏伟. 结核性胸膜炎患者外周血CD4 CD25 调节性T细胞水平及Foxp3 mRNA表达的分析[J]. 中国防痨杂志, 2009, 31(10): 569-572.

Lv Wei¹, Xue Keying², Wang Chengguo², Zhang Hongwei³. The analysis of CD4 CD25 regulatory T cells and Foxp3 mRNA in the peripheral blood from the patients with tuberculous pleurisy[J]. Chinese Journal of Antituberculosis, 2009, 31(10): 569-572.

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