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中国防痨杂志 ›› 2008, Vol. 30 ›› Issue (5): 424-426.

• 论著 • 上一篇    下一篇

TB-SA基因检测在结核病诊断的临床应用研究

朱帆；赵庆蓉；罗涛；曹玲；谢益新   

  1. 成都结核病防治院 成都 610061
  • 出版日期:2008-05-10 发布日期:2011-11-03

The clinical Application value of TB-SA genes of Mycobacterium tuberculosis for the tuberculosis diagnosis

Zhu Fan,Zhao Qingrong,Luo Tao, et al.   

  1. Chengdu Tuberculosis hospital Sichuan Province, Chengdu 610061, China
  • Online:2008-05-10 Published:2011-11-03

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摘要： 目的　研究结核分枝杆菌特异性基因(Tuberculosis-Specific AntigenTB-SA)在结核病诊断中的应用价值。方法 选择2004年4—9月期间在成都市结核病防治院的住院结核病患者371例。其中肺结核307例;肺外结核64例(结核性胸膜炎47例、结核性腹膜炎7例、结核性脑膜炎10例);非结核病的呼吸系统疾病患者61例(肺炎4例、肺癌9例,急性支气管炎7例、慢性支气管炎14例、哮喘10例,COPD12例、肺间质纤维化1例、支气管扩张3例、矽肺1例);同时选择无结核疾患健康志愿者40例,全部选例均知情同意。入选肺结核病例和健康选择痰液、肺外结核病例选择胸腔积液、腹腔积液、脑脊液等标本进行抗酸杆菌浓缩集菌,结核杆菌培养和TB-SA基因扩增(PCR)。结果 307例肺结核患者中PCR检测TB-SA阳性259例,敏感性为84.4%(259/307);细菌学培养阳性193例,敏感性62.9%(193/307);痰浓缩集菌镜检阳性95例,敏感性30.9%(95/307)。64例肺外结核患者中,TB-SA阳性35例;敏感性54.7%(35/64);核杆菌培养阳性5例,敏感性7.8%(5/64)。61例非结核呼吸系统病患者中,TB-SA阳性2例,特异性96.7%(59/61)痰涂片无阳性病例。40例健康志愿者,TB-SA及痰涂片均无阳性出现,特异性达100%(40/40)。结论 TB-SA基因检测的特异性及敏感性都取得了满意的效果。操作也较为简单。

关键词: 分枝杆菌,结核, 基因扩增(PCR), TB-SA基因, 结核/诊断

Abstract: Objective To study the application value of testing TB-SA genes of Mycobacterium tuberculosis in tuberculosis diagnosis. Methods Of 371 tuberculosis cases, 307 cases with pulmonary tuberculosis and 64 cases with nonpulmonary tuberculosis were selected. Sixty-one cases suffered from non-tuberculosis respiratory diseases such as pneumonia or lung cancer. Forty cases were selected as healthy control. The TB-SA gene was amplified by PCR, bacteriologic (smear and culture) using same sputum,thoracic,abdominal or cerebrospinal fluid. Result 259 of 307 cases PCR positive, sensation is 84.4% (259/307), and that the bacteriologic sensation is 62.5%(192/307). Among the 64 extra-pulmonary patients, TB-SA gene positive 35 cases, sensation is 54.7% (35/64); and the bacteriologic sensation is 7.8%(5/64). Meanwhile the result of 61 cases non-tuberculosis respiratory patients and the 40 healthiness, the especial are 96.7%(59/61) and 100%(40/40). Conclusion The especial and sensation of this kit was content. operating easily and could be apply popularly.

Key words: Mycobacterium tuberculosis, PCR, TB-SA genes, tuberculosis/diagnosis

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