肺结核患者sFas的测定及临床意义

中国防痨杂志 ›› 2005, Vol. 27 ›› Issue (4): 224-226.

肺结核患者sFas的测定及临床意义

刘加洪¹；王英年¹；宫希涛²；张春玲¹；许雁³；

1.山东省青岛市中心医院青岛 266042；2.青岛市胸科医院青岛 266042；3.宁阳县泗店医院宁阳 271400；

出版日期:2005-04-10 发布日期:2005-11-03

The mensuration and clinical values of the soluble Fas in tuberculosis patitents

Liu Jiahong, Wang Yingnian, Gong Xitao, et al.

The Research Institute of Tuberculosis and Respiratory Disease in Qingdao Center Hospital, Qingdao 266042, Chin

Online:2005-04-10 Published:2005-11-03

摘要/Abstract

摘要： 目的　测定肺结核患者外周血及外周血单个核细胞(peripheralbloodmononuclearcells,PBMC)培养上清液sFas含量,探讨其临床意义。方法用酶联免疫吸附实验(ELISA)测定63例肺结核外周血及其外周血单个核细胞(PBMC)培养72h上清液sFas的含量,选取35例正常人作为对照,进行临床分析。结果肺结核病人外周血及PBMC培养上清液sFas含量均显著高于正常对照组(分别为6.67±2.05/2.21±0.54；P＜0.01；46.64±12.44/15.97±3.51,P＜0.001)；有空洞病人高于无空洞病人(7.98±1.41/5.13±1.25,P＜0.01；55.94±18.52/35.73±11.44,P＜0.01)；治疗前病人高于治疗后病人(6.83±1.39/5.68±1.23P＜0.01；52.37±14.55/39.43±6.97,P＜0.01)。结论 sFas紊乱可能参与了肺结核的发病机制,其外周血含量的变化有可能作为肺结核病情判断、预后估计的实验室指标。

关键词: sFas, 结核,肺, 酶联免疫吸附实验

Abstract: Objective To determine the values and clinical significance of level of soluble Fas(sFas) in tuberculosis patitents . Methods The content of the sFas in the plasmas and the supernatant of peripheral blood mononuclear cells (PBMC) cultures of 63 tuberculosis patients were examined with enzyme-linked immuno- sorbent assay (ELISA) . ResultsThe levels of the sFas in both the plasmas and supernatant of PBMC cultures in tuberculosis patients were significantly higher than in controls (6.672.05/2.210.54;P＜0.01;46.6412.44/15.973.51,P＜0.001);The levels of the sFas in the plasmas and supernatant of PBMC cultures in patients with cavity were significantly higher than that in patients without cavity (7.981.41/5.131.25,P＜0.01;55.9418.52/35.7311.44,P＜0.01). The levels of the sFas in patients who had been treated more than one month were significatively higher than that in the patients who had not been treated (6.831.39/5.681.23 P＜0.01;52.3714.55/39.436.97,P＜0.01). ConclusionsThe disregulation of the sFas maybe a attribution factor to the occur of tuberculosis. The variety of the sFas in the patients with tuberculosis could be used to forecast the status and prognosis.

Key words: sFas, Pulmonary tuberculosis, Enzyme-linked immunosorbent assay(ELISA)

刘加洪,王英年,宫希涛,张春玲,许雁,. 肺结核患者sFas的测定及临床意义[J]. 中国防痨杂志, 2005, 27(4): 224-226.

Liu Jiahong, Wang Yingnian, Gong Xitao, et al.. The mensuration and clinical values of the soluble Fas in tuberculosis patitents[J]. Chinese Journal of Antituberculosis, 2005, 27(4): 224-226.

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