肺结核患者肿瘤坏死因子α基因多态性的研究

中国防痨杂志 ›› 2010, Vol. 32 ›› Issue (3): 155-159.

肺结核患者肿瘤坏死因子α基因多态性的研究

杨慧, 梁肇海, 冯铁建, 王峰

1.深圳市慢性病防治中心,深圳

出版日期:2010-03-20 发布日期:2010-03-20

-α gene polymorphisms in Chinese Han population of patients with pulmonary tub

Yang Hui,Liang Zhaohai,Feng Tiejian,Wang Feng

Shenzhen Center for Chronic Disease Control,Shenzhen 518020,China;2.Futian District,Shenzhen Center for Disease Control and Prevention,Shenzhen 518033,China

Online:2010-03-20 Published:2010-03-20
Contact: Yang Hui E-mail:yh2009cn@yahoo.com.cn

摘要/Abstract

摘要： 目的通过病例-对照研究，探讨肿瘤坏死因子-α（TNF-α）基因启动子区-238A/G、-308A/G位点单核苷酸多态性（SNP）与肺结核病的关系。方法采用序列特异性引物PCR（PCR-SSP）及测序技术检测深圳地区汉族人群肺结核患者200例及健康对照者197例TNF-α启动子区 -238A/G 、-308A/G位点基因多态性。采用直接计数法计算各组基因型频率及等位基因频率，并进行χ² 检验；采用SHEsis软件进行单倍型分析。以P值＜0.05为具有统计学意义。结果2组人群TNF-α启动子区-238 A/G、-308A/G位点基因型及等位基因分布频率差异无统计学意义(P>0.05)；两位点各种单倍型在2组间分布差异无统计学意义(P>0.05)。结论 TNF-α启动子区-238、-308位点基因多态性与中国汉族人群肺结核病易感性未见关联。

关键词: 结核, 肺, 肿瘤坏死因子-α, 多态性, 单核苷酸

Abstract: Objective To investigate the association of TNF-α gene promoter region polymorphisms at position -238A/G and -308A/G with susceptibility to pulmonary tuberculosis（PTB）by a case-control study. Methods Using polymerase chain reaction with sequence specific primer(PCR-SSP)/sequencing method, the single nucleotide polymorphisms (SNPs) of the promoter region of TNF-αgene at position -238 and -308 were detected in 200 pulmonary tuberculosis patients(group PTB) and 197 normal healthy individuals(group NHS) in the Han population from Shenzhen of China. The genotype frequencies and allele frequencies had been directly count out and χ² test was used to evaluate the difference of genotype frequencies distribution between two groups. Haplotypes were analyzed by SHEsis software (P=0.05). Results There was no significant difference in genotype frequencies and allele frequencies of TNF-αgene promoter region at position -238 (A/G) and -308(A/G) between two groups (P>0.05). There was no significant difference in haplotype frequencies between two groups (P>0.05). Conclusion s The polymorphisms at position -238 and -308 in promoter region of TNF-αgene in Chinese Han population may not be associated with susceptibility to pulmonary tuberculosis.

Key words: tuberculosis,pulmonary, tumor necrosis factor-alpha, polymorphism,single nucleotide

杨慧, 梁肇海, 冯铁建, 王峰. 肺结核患者肿瘤坏死因子α基因多态性的研究[J]. 中国防痨杂志, 2010, 32(3): 155-159.

Yang Hui,Liang Zhaohai,Feng Tiejian,Wang Feng. -α gene polymorphisms in Chinese Han population of patients with pulmonary tub[J]. Chinese Journal of Antituberculosis, 2010, 32(3): 155-159.

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