40例肺结核患者病情进展中白细胞介素-8表达变化的初步研究

中国防痨杂志 ›› 2013, Vol. 35 ›› Issue (9): 697-701.

40例肺结核患者病情进展中白细胞介素-8表达变化的初步研究

车南颖李雪莲张旭霞高孟秋李传友

101149 北京市结核病胸部肿瘤研究所首都医科大学附属北京胸科医院细菌免疫学研究室(车南颖、张旭霞、李传友)，结核内科(李雪莲、高孟秋)

收稿日期:2013-04-26 出版日期:2013-09-10 发布日期:2013-09-08
通信作者: 李传友 E-mail:lichuanyou6688@hotmail.com
基金资助:
国家自然科学基金（81101332）；北京市优秀人才培养资助项目（2010D003034000002）

Expression patterns of interleukin-8 during the progress of disease in 40 pulmonary tuberculosis patients

CHE Nan-ying, LI Xue-lian, ZHANG Xu-xia, GAO Meng-qiu, LI Chuan-you

Department of Bacteriology and Immunology, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China

Received:2013-04-26 Online:2013-09-10 Published:2013-09-08
Contact: LI Chuan-you E-mail:lichuanyou6688@hotmail.com

摘要/Abstract

摘要： 目的初步探索40例结核病患者外周血IL-8表达变化及其在肺结核病情进展中的表达变化。方法纳入对象为2011年5月至2013年2月首都医科大学附属北京胸科医院的40例初治肺结核患者，以同期本院15名健康体检者作为健康对照，并根据临床诊断信息对肺结核患者进行分组，将其中从诊断、治疗及治愈后复查阶段都拿到血液样品的8例肺结核患者，作治疗前后比较分析，纳入为跟踪调查组；按照细菌学及影像学检查结果，将另外32例分为病情缓和组（痰菌阴性、肺部无空洞，17例）与病情严重组（痰菌阳性、肺部有空洞，15例）。采集8例跟踪调查组患者治疗前、治疗中（治疗开始后2～3个月之间）和治疗结束后（停药后1～3个月之间）抗凝外周血；另外32例病情缓和组与病情严重组患者只收集治疗前抗凝外周血。加入结核分枝杆菌总蛋白刺激培养，再用酶联免疫吸附法检测血浆中的IL-8表达量。用SPSS 17.0统计软件进行Mann-Whitney U检验以分析健康对照组、病情缓和组及病情严重组患者之间IL-8表达是否存在差异，另外用Wilcoxon检验分析肺结核患者治疗前、治疗中及治疗后IL-8表达是否存在差异。IL-8检测数值用(x±s)表示，以P＜0.05为差异有统计学意义。结果经刺激诱导过的外周血IL-8表达量在病情严重组［(176.7±133.2) ng/ml］及病情缓和组［(39.6±52.2) ng/ml］均显著高于健康对照组［(7.1±6.3)ng/ml］(U=5, P=0.000 008; U=62, P=0.013)。并且病情严重组IL-8表达水平也显著高于病情缓和组 (U=37, P=0.001)。另外，肺结核患者治愈后IL-8表达水平［(5.9±3.6) ng/ml］与治疗前［(34.9±33.7) ng/ml］和治疗中［(51.7±34.2) ng/ml］相比有显著的降低 (Z=－2.240, P=0.025; Z=－2.521, P=0.012)。结论 IL-8表达水平与肺结核患者病情转归相关。

关键词: 结核, 肺/血液, 白细胞介素8, 疾病严重程度指数

Abstract: Objective To study the expression patterns of interleukin-8 (IL-8) in peripheral blood samples from 40 pulmonary tuberculosis (PTB) patients during progress of PTB. Methods Fifteen healthy donors and 40 newly diagnosed PTB patients were recruited from May 2011 to February 2013 in Beijing Chest Hospital. The PTB patients were divided into 3 groups: 8 patients for tracking studies; 17 patiens with mild symptoms (negative sputum bacteria, the lung without cavity); 15 patients with severe symptoms (positive sputum bacteria, the lung with cavity). Anticoagulant peripheral blood samples from 8 patients for tracking studies were collected before treatment, during treatment (2 to 3 months after treatment started), and after treatment (1 to 3 months after treatment ended), while that from the other 32 patients were collected only before treatment. Then the blood samples were incubated with Mycobacterium tuberculosis whole cell proteins. Plasma IL-8 expression levels were detected by enzyme linked immunosorbent assay. Statistical analysis was performed using SPSS 17.0 software. Mann-Whitney U test was used for analyzing IL-8 expression differences among healthy donors, mild PTB patients, and severe PTB patients. Wilcoxon test was used for comparing IL-8 expression levels before, during, and after treatment. IL-8 value was shown as (x±s), P value<0.05 was considered as statistically significant. Results Induced expression level of IL-8 in severe PTB patients was (176.7±133.2) ng/ml, while that in mild PTB patients was (39.6±52.2) ng/ml. Both expression levels were significantly higher than that in healthy donors ［(7.1±6.3) ng/ml］(U=5, P=0.000 008; U=62, P=0.013). Also, expression level of IL-8 in severe PTB patients was significantly higher than that in mild PTB patients (U=37, P=0.001). Additionally, IL-8 expression level in patients after treatment ［(5.9±3.6) ng/ml］was significantly decreased than those before treatment ［(34.9±33.7) ng/ml］and during treatment ［(51.7±34.2) ng/ml］(Z=－2.240, P=0.025; Z=－2.521, P=0.012). Conclusion IL-8 expression level is associated with progress of PTB.

Key words: Tuberculosis, pulmonary/blood, Interleukin-8, Severity of illness index

车南颖李雪莲张旭霞高孟秋李传友. 40例肺结核患者病情进展中白细胞介素-8表达变化的初步研究[J]. 中国防痨杂志, 2013, 35(9): 697-701.

CHE Nan-ying, LI Xue-lian, ZHANG Xu-xia, GAO Meng-qiu, LI Chuan-you. Expression patterns of interleukin-8 during the progress of disease in 40 pulmonary tuberculosis patients[J]. Chinese Journal of Antituberculosis, 2013, 35(9): 697-701.

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