39株对克拉霉素耐药的结核分枝杆菌临床分离株23S rRNA检测结果分析

中国防痨杂志 ›› 2013, Vol. 35 ›› Issue (9): 690-692.

39株对克拉霉素耐药的结核分枝杆菌临床分离株23S rRNA检测结果分析

邝小佳谭守勇吴碧彤蔡杏珊张院良

510095广州市胸科医院内四科

收稿日期:2013-05-23 出版日期:2013-09-10 发布日期:2013-09-08
通信作者: 邝小佳 E-mail:judy83573615@126.com

Analyses of 23S rRNAs of 39 clarithomycin-resistant Mycobacterium tuberculosis clinical isolates

KUANG Xiao-jia, TAN Shou-yong, WU Bi-tong, CAI Xing-shan, ZHANG Yuan-liang

Four Department of Internal Medicine，the Chest Hospital of Guangzhou，Guangzhou 510095，China

Received:2013-05-23 Online:2013-09-10 Published:2013-09-08
Contact: KUANG Xiao-jia E-mail:judy83573615@126.com

摘要/Abstract

摘要： 目的分析对克拉霉素耐药的结核分枝杆菌临床分离株23S rRNA的A2058位点的变化。方法选择我院菌株库的结核分枝杆菌临床分离株64株，其中10株为对全部抗结核药物敏感的结核分枝杆菌临床分离株；14株为单耐克拉霉素的结核分枝杆菌临床分离株；15株为耐多药，同时耐克拉霉素的结核分枝杆菌临床分离株；15株为耐多药，同时对克拉霉素敏感的结核分枝杆菌临床分离株；10株为广泛耐药菌株，同时对克拉霉素耐药的结核分枝杆菌临床分离株；此外，还有结核分枝杆菌标准株H37Rv 1株。对结核分枝杆菌23S rRNA行PCR检测和测序。结果经检测，H37Rv标准株没有A2058突变，只有1株广泛耐药临床分离株检测有A2058A-G的突变，其他临床分离株均没有突变，在耐克拉霉素的结核分枝杆菌临床分离株中占2.56%（1/39），在广泛耐药结核分枝杆菌临床分离株中占1/10。结论结核分枝杆菌临床分离株对克拉霉素耐药的机制中， A2058突变可能不是产生对克拉霉素耐药的主要机制。结核分枝杆菌产生对克拉霉素耐药的机制有待进一步研究。

关键词: Mycobacterium tuberculosis, Clarithromycin, RNA, ribosomal, 23S, Drug resistance, bacterial

Abstract: Objective To investigate the changes of 23S rRNA at site 2058 of clarithromycin-resistant Mycobacterium tuberculosis clinical isolates. Methods One Mycobacterium tuberculosis H37Rv standard strain and 64 isolates were detected, in which 39 clarithromycin-resistant isolates, 25 clarithromycin-sensitive isolates. The 23S rRNAs of Mycobacterium tuberculosis isolates were analyzed by polymerase chain reaction (PCR) and sequencing. Results The 23S rRNA of Mycobacterium tuberculosis H37Rv standard strain did not found the mutation at site 2058. Of 64 Mycobacterium tuberculosis isolates, only 1 extensively drug-resistant (XDR) strain had A2058A-G mutation of 23S rRNA, which account 2.56%（1/39） clarithromycin-resistant strains and 1/10 XDR strains. Conclusion The mutation of 23S rRNA at site 2058 of Mycobacterium tuberculosis may not be the main cause inducing its clarithromycin resistance. The mechanism of Mycobacterium tuberculosis clarithromycin resistance need be further studied.

Key words: Mycobacterium tuberculosis, Clarithromycin, RNA, ribosomal, 23S, Drug resistance, bacterial

邝小佳谭守勇吴碧彤蔡杏珊张院良. 39株对克拉霉素耐药的结核分枝杆菌临床分离株23S rRNA检测结果分析[J]. 中国防痨杂志, 2013, 35(9): 690-692.

KUANG Xiao-jia, TAN Shou-yong, WU Bi-tong, CAI Xing-shan, ZHANG Yuan-liang. Analyses of 23S rRNAs of 39 clarithomycin-resistant Mycobacterium tuberculosis clinical isolates[J]. Chinese Journal of Antituberculosis, 2013, 35(9): 690-692.

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