sICAM-1、sIL-2R诊断肺结核最佳截值点的初步研究

中国防痨杂志 ›› 2009, Vol. 31 ›› Issue (4): 181-184.

• 论著 • 下一篇

sICAM-1、sIL-2R诊断肺结核最佳截值点的初步研究

张瑾¹；洪志强²；黄惠良²；崔喜文²；沈月平³

1. 江苏省昆山市第一人民医院昆山 215300；2. 江苏省昆山市疾病控制中心昆山 215301；3.江苏省苏州大学放射医学与公共卫生学院苏州215325

出版日期:2009-04-10 发布日期:2011-11-03
基金资助:
昆山市科技局2006年社会发展科技计划项目的资助（项目编号：KS0626）

Optimum cutoff value of the sICAM-1, sIL-2R level for the diagnosis of the pulmonary tuberculosis

Zhang Jin¹, Hong Zhiqiang², Huang Huiliang², Cui Xiwen², Shen Yueping³

1.The first people’s hospital of Kunshan, Jiangsu Province, Kunshan 215300, China;2.The center for disease control and prevention of Kunshan, Jiangsu Province, Kunshan 215301, China;3.The Radiomedicine and public health institute of Suzhou University, Jiangsu Province, Suzhou 215325, China

Online:2009-04-10 Published:2011-11-03

摘要/Abstract

摘要： 目的研究sICAM-1、sIL-2R在肺结核菌阳、菌阴及健康对照人群中的相关性，探讨sICAM-1与sIL-2R指标诊断结核病的最优灵敏度和特异度的截值点。方法采用酶联免疫吸附法（ELISA）测定97例在江苏省昆山市第一人民医院及乡镇医院就诊年龄在20～60岁的新发肺结核患者（53例菌阳、44例菌阴）及经年龄频数（±3岁）、同性别匹配的93例正常健康人的血清sICAM-1、sIL-2R水平。计算sICAM-1、sIL-2R在肺结核菌阳、菌阴及健康对照人群中的pearson相关系数，采用ROC曲线探索sICAM-1、sIL-2R诊断结核病的最优灵敏度和特异度的截值点。结果在菌阴患者中，sICAM-1与sIL-2R呈显著的正相关关系（r=0.4639, P=0.0017）。而菌阳患者和健康人群中，两者无明显的线性相关关系，相关系数分别为0.0472(P=0.7397)、-0.0077(P=0.9419)。sICAM-1诊断结核病的最优截值点为：364.04ng/ml,　灵敏度为83.0%, 特异度为83.9%，sIL-2R诊断结核病的最优截值点为：2.49ng/ml,，灵敏度为63.9%, 特异度为64.8%。结论sICAM-1水平≥364.04ng/ml可作为诊断结核病的一个辅助参考指标。

关键词: 结核, 肺/诊断, 胞间黏附分子1, 受体, 白细胞介素2

Abstract: ObjectiveTo explore the correlation between the serum level of soluble intercellular adhesion molecule-1 (sICAM-1) and soluble interleukin-2 receptor (sIL-2R) in the smear-positive, -negative patients with pulmonary tuberculosis (TB) and health control, and to develop the optimum cutoff values of sICAM-1 and sIL-2R for the diagnosis of the TB patients. MethodELISA assay was used to measure the serum level of sICAM-1, sIL-2R in 97 newly diagnosed TB cases (containing 53 smear-positive cases, 44 smear-negative cases) aged 20 to 60 years old and being treated in Kunshan first people hospital and the township-level hospitals, Jiangsu province and 93 health controls matched by ages (±3 yrs) and sex. The pearson correlation coefficient r was estimated between the sICAM-1 and sIL-2R level among the smear-positive, smear-negative and control groups. ROC curve was used to explore the optimum cutoff value of sICAM-1, sIL-2R for the diagnosis of the TB. Resultsthe serum level of sICAM-1 was positively associated with level of sIL-2R in the smear-negative cases（r=0.4639, P=0.0017）. No significant linear correlations were found between the two indexes among the smear-positive TB patients and control population (r₁=0.0472, P=0.7397, r₂=-0.0077, P=0.9419). When the cutoff value of sICAM-1 level was higher than 364.04ng/ml, its sensitivity and specificity for the diagnosis of TB were 83.0% and 83.9%, respectively. For sIL-2R with cutoff value lower than 2.49ng/ml, the sensitivity and specificity were 63.9% and 64.8%, respectively. ConclusionThe sICAM-1 level higher than 364.04ng/ml could be regarded as the clinical diagnosis reference value for TB.

Key words: tuberculosis,pulmonary/diagnosis, intercellular adhesion molecule-1, receptors,interleukin-2

张瑾,洪志强,黄惠良,崔喜文,沈月平. sICAM-1、sIL-2R诊断肺结核最佳截值点的初步研究[J]. 中国防痨杂志, 2009, 31(4): 181-184.

Zhang Jin¹, Hong Zhiqiang², Huang Huiliang², Cui Xiwen², Shen Yueping³. Optimum cutoff value of the sICAM-1, sIL-2R level for the diagnosis of the pulmonary tuberculosis[J]. Chinese Journal of Antituberculosis, 2009, 31(4): 181-184.

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sICAM-1、sIL-2R诊断肺结核最佳截值点的初步研究

Optimum cutoff value of the sICAM-1, sIL-2R level for the diagnosis of the pulmonary tuberculosis

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